Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo
Hair follicle stem cells (HFSCs) are potentially useful for the treatment of skin injuries and diseases. To achieve clinical application, a prerequisite must be accomplished: harvesting enough HFSCs from limited skin biopsy. The commonly used sorting approach for isolating HFSCs, however, suffers fr...
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2012-06-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/096368912X640538 |
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doaj-94185af869234ffb92b6b565131635eb2020-11-25T03:01:43ZengSAGE PublishingCell Transplantation0963-68971555-38922012-06-012110.3727/096368912X640538Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in VivoShoubing Zhang0Huimin Hu1Huishan Zhang2Shuang Liu3Shu Liu4Ying Zhang5Xiaohua Lei6Lina Ning7Yujing Cao8Enkui Duan9Graduate University of the Chinese Academy of Sciences, Beijing, ChinaGraduate University of the Chinese Academy of Sciences, Beijing, ChinaGraduate University of the Chinese Academy of Sciences, Beijing, ChinaGraduate University of the Chinese Academy of Sciences, Beijing, ChinaGraduate University of the Chinese Academy of Sciences, Beijing, ChinaGraduate University of the Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaHair follicle stem cells (HFSCs) are potentially useful for the treatment of skin injuries and diseases. To achieve clinical application, a prerequisite must be accomplished: harvesting enough HFSCs from limited skin biopsy. The commonly used sorting approach for isolating HFSCs, however, suffers from its intrinsic disadvantages, such as requirement of large-scale skin biopsy. Here, we report an efficient organ culture method to isolate and expand rat HFSCs from limited skin biopsy and these HFSCs could reconstitute the epidermis and the hair follicles (HFs). Seventy-three percent of cultured HFs formed hair follicle stem cell colonies from the bulge, and a single hair follicle provided all the HFSCs used in this research, demonstrating the high efficiency of this method. Quantitative RT-PCR and immunofluorescent staining results revealed that these stem cells obtained from the bulge highly expressed basal layer markers K14 and alpha-6 integrin, epithelial stem cell marker P63, and bulge stem cell marker K15. After long-term culture in vitro, GFP-labeled hair follicle stem cells formed new hair follicles, epidermis, and sebaceous glands following xenotransplantation into the back of nude mice. This study indicated that multipotent hair follicle stem cells could be efficiently harvested through organ culture from limited skin material—even a single hair follicle—and reconstitute hair follicles in vivo after long-term expansion culture, providing the basis for future clinical applications.https://doi.org/10.3727/096368912X640538 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shoubing Zhang Huimin Hu Huishan Zhang Shuang Liu Shu Liu Ying Zhang Xiaohua Lei Lina Ning Yujing Cao Enkui Duan |
spellingShingle |
Shoubing Zhang Huimin Hu Huishan Zhang Shuang Liu Shu Liu Ying Zhang Xiaohua Lei Lina Ning Yujing Cao Enkui Duan Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo Cell Transplantation |
author_facet |
Shoubing Zhang Huimin Hu Huishan Zhang Shuang Liu Shu Liu Ying Zhang Xiaohua Lei Lina Ning Yujing Cao Enkui Duan |
author_sort |
Shoubing Zhang |
title |
Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo |
title_short |
Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo |
title_full |
Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo |
title_fullStr |
Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo |
title_full_unstemmed |
Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo |
title_sort |
hair follicle stem cells derived from single rat vibrissa via organ culture reconstitute hair follicles in vivo |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2012-06-01 |
description |
Hair follicle stem cells (HFSCs) are potentially useful for the treatment of skin injuries and diseases. To achieve clinical application, a prerequisite must be accomplished: harvesting enough HFSCs from limited skin biopsy. The commonly used sorting approach for isolating HFSCs, however, suffers from its intrinsic disadvantages, such as requirement of large-scale skin biopsy. Here, we report an efficient organ culture method to isolate and expand rat HFSCs from limited skin biopsy and these HFSCs could reconstitute the epidermis and the hair follicles (HFs). Seventy-three percent of cultured HFs formed hair follicle stem cell colonies from the bulge, and a single hair follicle provided all the HFSCs used in this research, demonstrating the high efficiency of this method. Quantitative RT-PCR and immunofluorescent staining results revealed that these stem cells obtained from the bulge highly expressed basal layer markers K14 and alpha-6 integrin, epithelial stem cell marker P63, and bulge stem cell marker K15. After long-term culture in vitro, GFP-labeled hair follicle stem cells formed new hair follicles, epidermis, and sebaceous glands following xenotransplantation into the back of nude mice. This study indicated that multipotent hair follicle stem cells could be efficiently harvested through organ culture from limited skin material—even a single hair follicle—and reconstitute hair follicles in vivo after long-term expansion culture, providing the basis for future clinical applications. |
url |
https://doi.org/10.3727/096368912X640538 |
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