In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran
Background: Members of the genus Centaurea L. (Astraceae) are used in some countries for betterment of various ailments in the popular medicine. The main group of secondary metabolites in plants are phenolic compounds and flavonoids which are the main subset of phenolic compounds, possess a lot of b...
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Tabriz University of Medical Sciences
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doaj-943f6bb871384f4c82b6f38fe39ddd1a2021-02-02T05:25:32ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862383-28862017-12-0123431632310.15171/PS.2017.46In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from IranRoya Karamian*, Rose Eghbalian Background: Members of the genus Centaurea L. (Astraceae) are used in some countries for betterment of various ailments in the popular medicine. The main group of secondary metabolites in plants are phenolic compounds and flavonoids which are the main subset of phenolic compounds, possess a lot of biological properties such as antioxidant activity. The aim of present research is to determine in vitro total phenol and flavonoid contents, antioxidant potency and also antibacterial activity of the methanolic extracts of capitulla and aerial parts of three Centaurea species, namely C. sosnovsky Grossh., C. irritans Wagenitz and C. kandavanesis Wagenitz. In addition, any relationships between the phenolics content and their antioxidant properties were discussed. Methods: To determine total phenol and flavonoid contents of the methanolic extracts, Folin-Ciocalteu and aluminum chloride methods were used, respectively. To evaluate antioxidant activity three different test systems, namely 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging (DPPH), Cu-chelating and ß-carotene/linoleic acid bleaching were used. In addition, disc diffusion method was used to assess antibacterial activity of the extracts against 6 gram positive and negative bacteria. Results: Results indicated that the highest total phenol content (98.91 ± 1.87mg GAE/g of dry extract) represented by the aerial part extract of C. kandavanensis. The highest total flavonoid content (5.382 ± 0.231mg GAE/g of dry extract) and also DPPH radical scavenging activity (IC50; 0.20 ± 2.02 mg/ml) were exhibited by the capitulla extract of C. sosnovsky. However, the aerial part extract of C. irritans had the most potency for β-carotene bleaching (74.74%) among the studied samples. In addition, the extracts had acceptable antibacterial activity against both gram-positive and gram-negative bacteria tested. Conclusion: Overall, high content of phenolic compounds and proper antioxidant activity of the examined Centaurea extracts may suggest them as potent antioxidants for special use in future. http://journals.tbzmed.ac.ir/PHARM/Abstract/PHARM_3390_20170122185446Antibacterial activityAntioxidantCentaureaDPPH radical scavenging;FlavonoidPhenol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roya Karamian*, Rose Eghbalian |
spellingShingle |
Roya Karamian*, Rose Eghbalian In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran Pharmaceutical Sciences Antibacterial activity Antioxidant Centaurea DPPH radical scavenging; Flavonoid Phenol |
author_facet |
Roya Karamian*, Rose Eghbalian |
author_sort |
Roya Karamian*, Rose Eghbalian |
title |
In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran |
title_short |
In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran |
title_full |
In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran |
title_fullStr |
In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran |
title_full_unstemmed |
In Vitro Evaluation of Phenolics Content, Antioxidant and Antimicrobial Activities of Three Centaurea L. Species from Iran |
title_sort |
in vitro evaluation of phenolics content, antioxidant and antimicrobial activities of three centaurea l. species from iran |
publisher |
Tabriz University of Medical Sciences |
series |
Pharmaceutical Sciences |
issn |
2383-2886 2383-2886 |
publishDate |
2017-12-01 |
description |
Background: Members of the genus Centaurea L. (Astraceae) are used in some countries for betterment of various ailments in the popular medicine. The main group of secondary metabolites in plants are phenolic compounds and flavonoids which are the main subset of phenolic compounds, possess a lot of biological properties such as antioxidant activity. The aim of present research is to determine in vitro total phenol and flavonoid contents, antioxidant potency and also antibacterial activity of the methanolic extracts of capitulla and aerial parts of three Centaurea species, namely C. sosnovsky Grossh., C. irritans Wagenitz and C. kandavanesis Wagenitz. In addition, any relationships between the phenolics content and their antioxidant properties were discussed. Methods: To determine total phenol and flavonoid contents of the methanolic extracts, Folin-Ciocalteu and aluminum chloride methods were used, respectively. To evaluate antioxidant activity three different test systems, namely 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging (DPPH), Cu-chelating and ß-carotene/linoleic acid bleaching were used. In addition, disc diffusion method was used to assess antibacterial activity of the extracts against 6 gram positive and negative bacteria. Results: Results indicated that the highest total phenol content (98.91 ± 1.87mg GAE/g of dry extract) represented by the aerial part extract of C. kandavanensis. The highest total flavonoid content (5.382 ± 0.231mg GAE/g of dry extract) and also DPPH radical scavenging activity (IC50; 0.20 ± 2.02 mg/ml) were exhibited by the capitulla extract of C. sosnovsky. However, the aerial part extract of C. irritans had the most potency for β-carotene bleaching (74.74%) among the studied samples. In addition, the extracts had acceptable antibacterial activity against both gram-positive and gram-negative bacteria tested. Conclusion: Overall, high content of phenolic compounds and proper antioxidant activity of the examined Centaurea extracts may suggest them as potent antioxidants for special use in future.
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topic |
Antibacterial activity Antioxidant Centaurea DPPH radical scavenging; Flavonoid Phenol |
url |
http://journals.tbzmed.ac.ir/PHARM/Abstract/PHARM_3390_20170122185446 |
work_keys_str_mv |
AT royakaramianroseeghbalian invitroevaluationofphenolicscontentantioxidantandantimicrobialactivitiesofthreecentaurealspeciesfromiran |
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1724303746378235904 |