Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-...

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Main Authors: Sho Yoshimatsu, Rei Murakami, Tsukika Sato, Tsubasa Saeki, Masafumi Yamamoto, Erika Sasaki, Toshiaki Noce, Hideyuki Okano
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001549
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spelling doaj-94498ae9d4ff401ea20b4cbb9717933a2021-05-30T04:41:28ZengElsevierStem Cell Research1873-50612021-05-0153102308Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter alleleSho Yoshimatsu0Rei Murakami1Tsukika Sato2Tsubasa Saeki3Masafumi Yamamoto4Erika Sasaki5Toshiaki Noce6Hideyuki Okano7Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, JapanICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, JapanDepartment of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, JapanLaboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, JapanDepartment of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Corresponding author.POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).http://www.sciencedirect.com/science/article/pii/S1873506121001549
collection DOAJ
language English
format Article
sources DOAJ
author Sho Yoshimatsu
Rei Murakami
Tsukika Sato
Tsubasa Saeki
Masafumi Yamamoto
Erika Sasaki
Toshiaki Noce
Hideyuki Okano
spellingShingle Sho Yoshimatsu
Rei Murakami
Tsukika Sato
Tsubasa Saeki
Masafumi Yamamoto
Erika Sasaki
Toshiaki Noce
Hideyuki Okano
Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
Stem Cell Research
author_facet Sho Yoshimatsu
Rei Murakami
Tsukika Sato
Tsubasa Saeki
Masafumi Yamamoto
Erika Sasaki
Toshiaki Noce
Hideyuki Okano
author_sort Sho Yoshimatsu
title Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
title_short Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
title_full Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
title_fullStr Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
title_full_unstemmed Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
title_sort generation of a common marmoset embryonic stem cell line cmes40-oc harboring a pou5f1 (oct4)-2a-mcerulean3 knock-in reporter allele
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).
url http://www.sciencedirect.com/science/article/pii/S1873506121001549
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