CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates
Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-07-01
|
Series: | Frontiers in Genetics |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00260/full |
id |
doaj-944bf3f3da9340439ce8199fd9bfcf04 |
---|---|
record_format |
Article |
spelling |
doaj-944bf3f3da9340439ce8199fd9bfcf042020-11-24T23:08:57ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-07-01910.3389/fgene.2018.00260385330CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid VertebratesFanqian Yin0Fanqian Yin1Wenfu Liu2Wenfu Liu3Jing Chai4Jing Chai5Bin Lu6Bin Lu7Robert W. Murphy8Robert W. Murphy9Jing Luo10Jing Luo11School of Life Sciences, Yunnan University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaSchool of Life Sciences, Yunnan University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaSchool of Life Sciences, Yunnan University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaSchool of Life Sciences, Yunnan University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaState Key Laboratory of Genetic Resources and Evolution, Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, ChinaCentre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, ON, CanadaSchool of Life Sciences, Yunnan University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, ChinaPolyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes. Investigations into this require the identification of genes related to polyploidization and the discrimination of dosage-balance from paternal and maternal copies, and regardless of the mechanism being either autopolyploidization or allopolyploidization. New approaches and technologies may discern the mosaic of novel gene functions gained through the recombination of paternal and maternal genes in allopolyploidization. Modifications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with CRISPR-associated system (Cas) protein 9 (CRISPR/Cas9) have been employed in studies of polyploidization of plants. However, the approach has seldom been applied to polyploidization in vertebrates. Herein, we use CRISPR/Cas9 to trace gene-fate in tetraploid goldfish, and specifically to identify the functional differentiation of two divergent copies of fgf20a, which are expressed differently throughout embryonic development. We expect this gene editing system will be applicable to studies of polyploids and the genetic improvement of polyploid livestock.https://www.frontiersin.org/article/10.3389/fgene.2018.00260/fullpolyploidyCRISPR/Cas9gene editinggene-fategene copies |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fanqian Yin Fanqian Yin Wenfu Liu Wenfu Liu Jing Chai Jing Chai Bin Lu Bin Lu Robert W. Murphy Robert W. Murphy Jing Luo Jing Luo |
spellingShingle |
Fanqian Yin Fanqian Yin Wenfu Liu Wenfu Liu Jing Chai Jing Chai Bin Lu Bin Lu Robert W. Murphy Robert W. Murphy Jing Luo Jing Luo CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates Frontiers in Genetics polyploidy CRISPR/Cas9 gene editing gene-fate gene copies |
author_facet |
Fanqian Yin Fanqian Yin Wenfu Liu Wenfu Liu Jing Chai Jing Chai Bin Lu Bin Lu Robert W. Murphy Robert W. Murphy Jing Luo Jing Luo |
author_sort |
Fanqian Yin |
title |
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates |
title_short |
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates |
title_full |
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates |
title_fullStr |
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates |
title_full_unstemmed |
CRISPR/Cas9 Application for Gene Copy Fate Survey of Polyploid Vertebrates |
title_sort |
crispr/cas9 application for gene copy fate survey of polyploid vertebrates |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2018-07-01 |
description |
Polyploidization occurs widely in eukaryotes, and especially in plants. Polyploid plants and some fishes have been commercialized. Typically, severe genomic perturbations immediately follow polyploidization and little is known about how polyploid offspring survives the genetic and epigenetic changes. Investigations into this require the identification of genes related to polyploidization and the discrimination of dosage-balance from paternal and maternal copies, and regardless of the mechanism being either autopolyploidization or allopolyploidization. New approaches and technologies may discern the mosaic of novel gene functions gained through the recombination of paternal and maternal genes in allopolyploidization. Modifications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with CRISPR-associated system (Cas) protein 9 (CRISPR/Cas9) have been employed in studies of polyploidization of plants. However, the approach has seldom been applied to polyploidization in vertebrates. Herein, we use CRISPR/Cas9 to trace gene-fate in tetraploid goldfish, and specifically to identify the functional differentiation of two divergent copies of fgf20a, which are expressed differently throughout embryonic development. We expect this gene editing system will be applicable to studies of polyploids and the genetic improvement of polyploid livestock. |
topic |
polyploidy CRISPR/Cas9 gene editing gene-fate gene copies |
url |
https://www.frontiersin.org/article/10.3389/fgene.2018.00260/full |
work_keys_str_mv |
AT fanqianyin crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT fanqianyin crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT wenfuliu crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT wenfuliu crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT jingchai crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT jingchai crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT binlu crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT binlu crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT robertwmurphy crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT robertwmurphy crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT jingluo crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates AT jingluo crisprcas9applicationforgenecopyfatesurveyofpolyploidvertebrates |
_version_ |
1725612262620659712 |