Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes
The roles of calmodulin (CaM)-dependent protein kinase II (CaMKII) in the maintenance of basal activity and the reversion of run-down of L-type Ca2+ channels were studied in guinea-pig ventricular myocytes by the patch-clamp technique. In the cell-attached configuration, the Ca2+-channel activity wa...
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doaj-946b2934b835407cbd79c37af208e9542020-11-25T02:06:26ZengElsevierJournal of Pharmacological Sciences1347-86132008-01-011083290300Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular MyocytesLi-Ying Hao0Jian-Jun Xu1Etsuko Minobe2Asako Kameyama3Masaki Kameyama4Department of Pharmaceutical Toxicology, School of Pharmacological Sciences, China Medical University, Shenyang 110001, China; Department of Physiology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan; Corresponding author (affiliation #1). lyhao@mail.cmu.edu.cnDepartment of Physiology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, JapanDepartment of Physiology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, JapanDepartment of Physiology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, JapanDepartment of Physiology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, JapanThe roles of calmodulin (CaM)-dependent protein kinase II (CaMKII) in the maintenance of basal activity and the reversion of run-down of L-type Ca2+ channels were studied in guinea-pig ventricular myocytes by the patch-clamp technique. In the cell-attached configuration, the Ca2+-channel activity was inhibited to 82% – 26% by 1 – 10 μM KN-93 and to 92% – 66% by 0.1 – 1 μM autocamtide-2–related inhibitory peptide (AIP) myristoylated. In the inside-out configuration, the bovine cardiac cytoplasm recovered Ca2+-channel activity to 87% of that recorded in the cell-attached configuration, while the CaMKII inhibitor 281-301 at 10 μM reduced the recovery effect to 19%. CaM + ATP recovered the channel activity to 93% and 28% of that recorded in the cell-attached configuration when applied at 1 and 5 min after run-down, respectively, showing a time-dependent attenuation. However, in the presence of 0. 33 μM CaMKII, this attenuation was abolished, showing 85% and 75% recovery when applied at 1 and 5 min after run-down, respectively. This recovery effect was suppressed by 10 μM AIP, applied at 5 min, but not at 1 min after run-down. We concluded that CaMKII activation is required in the maintenance of basal activity of L-type Ca2+ channels. Keywords:: calmodulin-dependent protein kinase II (CaMKII), calmodulin, cardiac myocyte, L-type Ca2+ channelhttp://www.sciencedirect.com/science/article/pii/S1347861319313362 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-Ying Hao Jian-Jun Xu Etsuko Minobe Asako Kameyama Masaki Kameyama |
spellingShingle |
Li-Ying Hao Jian-Jun Xu Etsuko Minobe Asako Kameyama Masaki Kameyama Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes Journal of Pharmacological Sciences |
author_facet |
Li-Ying Hao Jian-Jun Xu Etsuko Minobe Asako Kameyama Masaki Kameyama |
author_sort |
Li-Ying Hao |
title |
Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes |
title_short |
Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes |
title_full |
Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes |
title_fullStr |
Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes |
title_full_unstemmed |
Calmodulin Kinase II Activation Is Required for the Maintenance of Basal Activity of L-Type Ca2+ Channels in Guinea-Pig Ventricular Myocytes |
title_sort |
calmodulin kinase ii activation is required for the maintenance of basal activity of l-type ca2+ channels in guinea-pig ventricular myocytes |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2008-01-01 |
description |
The roles of calmodulin (CaM)-dependent protein kinase II (CaMKII) in the maintenance of basal activity and the reversion of run-down of L-type Ca2+ channels were studied in guinea-pig ventricular myocytes by the patch-clamp technique. In the cell-attached configuration, the Ca2+-channel activity was inhibited to 82% – 26% by 1 – 10 μM KN-93 and to 92% – 66% by 0.1 – 1 μM autocamtide-2–related inhibitory peptide (AIP) myristoylated. In the inside-out configuration, the bovine cardiac cytoplasm recovered Ca2+-channel activity to 87% of that recorded in the cell-attached configuration, while the CaMKII inhibitor 281-301 at 10 μM reduced the recovery effect to 19%. CaM + ATP recovered the channel activity to 93% and 28% of that recorded in the cell-attached configuration when applied at 1 and 5 min after run-down, respectively, showing a time-dependent attenuation. However, in the presence of 0. 33 μM CaMKII, this attenuation was abolished, showing 85% and 75% recovery when applied at 1 and 5 min after run-down, respectively. This recovery effect was suppressed by 10 μM AIP, applied at 5 min, but not at 1 min after run-down. We concluded that CaMKII activation is required in the maintenance of basal activity of L-type Ca2+ channels. Keywords:: calmodulin-dependent protein kinase II (CaMKII), calmodulin, cardiac myocyte, L-type Ca2+ channel |
url |
http://www.sciencedirect.com/science/article/pii/S1347861319313362 |
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