De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs
A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recogn...
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doaj-947cc1e6325342e9b9eaa16309e14a212020-11-25T03:57:44ZengMDPI AGBiomolecules2218-273X2020-10-01101470147010.3390/biom10111470De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from MolluscsPavlina Dolashka0Asya Daskalova1Aleksandar Dolashki2Wolfgang Voelter3Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInterfacultary Institute of Biochemistry, University of Tuebingen, 72074 Tuebingen, GermanyA number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recognition. The large amount of scientific interest in glycoproteins of molluscan hemocyanins is due not only to their complex quaternary structures, but also to the great diversity of their oligosaccharide structures with a high carbohydrate content (2–9%). This great variety is due to their specific monosaccharide composition and different side chain composition. The determination of glycans and glycopeptides was performed with the most commonly used methods for the analysis of biomolecules, including peptides and proteins, including Matrix Assisted Laser Desorption/Ionisation–Time of Flight (MALDI-TOF-TOF), Liquid Chromatography - Electrospray Ionization-Mass Spectrometry (LC/ESI-MS), Liquid Chromatography (LC-Q-trap-MS/MS) or Nano- Electrospray Ionization-Mass Spectrometry (nano-ESI-MS) and others. The molluscan hemocyanins have complex carbohydrate structures with predominant <b><i>N</i></b>-linked glycans. Of interest are identified structures with methylated hexoses and xyloses arranged at different positions in the carbohydrate moieties of molluscan hemocyanins. Novel acidic glycan structures with specific glycosylation positions, e.g., hemocyanins that enable a deeper insight into the glycosylation process, were observed in <i>Rapana venosa</i>, <i>Helix lucorum</i>, and <i>Haliotis tuberculata</i>. Recent studies demonstrate that glycosylation plays a crucial physiological role in the immunostimulatory and therapeutic effect of glycoproteins. The remarkable diversity of hemocyanin glycan content is an important feature of their immune function and provides a new concept in the antibody–antigen interaction through clustered carbohydrate epitopes.https://www.mdpi.com/2218-273X/10/11/1470hemocyaninsmolluscsmass spectrometric analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pavlina Dolashka Asya Daskalova Aleksandar Dolashki Wolfgang Voelter |
spellingShingle |
Pavlina Dolashka Asya Daskalova Aleksandar Dolashki Wolfgang Voelter De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs Biomolecules hemocyanins molluscs mass spectrometric analysis |
author_facet |
Pavlina Dolashka Asya Daskalova Aleksandar Dolashki Wolfgang Voelter |
author_sort |
Pavlina Dolashka |
title |
De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_short |
De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_full |
De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_fullStr |
De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_full_unstemmed |
De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs |
title_sort |
de novo structural determination of the oligosaccharide structure of hemocyanins from molluscs |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2020-10-01 |
description |
A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recognition. The large amount of scientific interest in glycoproteins of molluscan hemocyanins is due not only to their complex quaternary structures, but also to the great diversity of their oligosaccharide structures with a high carbohydrate content (2–9%). This great variety is due to their specific monosaccharide composition and different side chain composition. The determination of glycans and glycopeptides was performed with the most commonly used methods for the analysis of biomolecules, including peptides and proteins, including Matrix Assisted Laser Desorption/Ionisation–Time of Flight (MALDI-TOF-TOF), Liquid Chromatography - Electrospray Ionization-Mass Spectrometry (LC/ESI-MS), Liquid Chromatography (LC-Q-trap-MS/MS) or Nano- Electrospray Ionization-Mass Spectrometry (nano-ESI-MS) and others. The molluscan hemocyanins have complex carbohydrate structures with predominant <b><i>N</i></b>-linked glycans. Of interest are identified structures with methylated hexoses and xyloses arranged at different positions in the carbohydrate moieties of molluscan hemocyanins. Novel acidic glycan structures with specific glycosylation positions, e.g., hemocyanins that enable a deeper insight into the glycosylation process, were observed in <i>Rapana venosa</i>, <i>Helix lucorum</i>, and <i>Haliotis tuberculata</i>. Recent studies demonstrate that glycosylation plays a crucial physiological role in the immunostimulatory and therapeutic effect of glycoproteins. The remarkable diversity of hemocyanin glycan content is an important feature of their immune function and provides a new concept in the antibody–antigen interaction through clustered carbohydrate epitopes. |
topic |
hemocyanins molluscs mass spectrometric analysis |
url |
https://www.mdpi.com/2218-273X/10/11/1470 |
work_keys_str_mv |
AT pavlinadolashka denovostructuraldeterminationoftheoligosaccharidestructureofhemocyaninsfrommolluscs AT asyadaskalova denovostructuraldeterminationoftheoligosaccharidestructureofhemocyaninsfrommolluscs AT aleksandardolashki denovostructuraldeterminationoftheoligosaccharidestructureofhemocyaninsfrommolluscs AT wolfgangvoelter denovostructuraldeterminationoftheoligosaccharidestructureofhemocyaninsfrommolluscs |
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