Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients
Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed fro...
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doaj-949d4f40e45d49a9ad769077ae4357ed2020-11-25T00:23:27ZengMDPI AGJournal of Clinical Medicine2077-03832019-07-018798810.3390/jcm8070988jcm8070988Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted PatientsJonas Abo Basha0Matthias Kiel1Dennis Görlich2Katharina Schütte-Nütgen3Anika Witten4Hermann Pavenstädt5Barbara C. Kahl6Ulrich Dobrindt7Stefan Reuter8Department of Medicine D, Division of General Internal Medicine, Nephrology and Rheumatology, University Hospital Münster, 48149 Münster, GermanyInstitute of Hygiene, University of Münster, 48149 Münster, GermanyInstitute of Biostatistics and Clinical Research, University of Münster, 48149 Münster, GermanyDepartment of Medicine D, Division of General Internal Medicine, Nephrology and Rheumatology, University Hospital Münster, 48149 Münster, GermanyInstitute for Human Genetics, University of Münster, 48149 Münster, GermanyDepartment of Medicine D, Division of General Internal Medicine, Nephrology and Rheumatology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Hygiene, University of Münster, 48149 Münster, GermanyDepartment of Medicine D, Division of General Internal Medicine, Nephrology and Rheumatology, University Hospital Münster, 48149 Münster, GermanyUrinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed from the UPEC of non-KTx patients. Therefore, we determined the genome sequences of 182 UPEC isolates from KTx and control patients in a large German university clinic and pheno- and genotypically compared these two isolated groups. Resistance to the β-lactams, trimethoprim or trimethoprim/sulfamethoxazole was significantly higher among UPEC from KTx than from control patients, whereas both the isolated groups were highly susceptible to fosfomycin. Accordingly, the gene content conferring resistance to β-lactams or trimethoprim, but also to aminoglycosides, was significantly higher in KTx than in control UPEC isolates. <i>E. coli</i> isolates from KTx patients more frequently presented with uncommon UPEC phylogroups expressing higher numbers of plasmid replicons, but interestingly, less UPEC virulence-associated genes than the control group. We conclude that there is no defining subset of virulence traits for UPEC from KTx patients. The clinical history and immunocompromised status of KTx patients enables <i>E. coli</i> strains with low uropathogenic potential, but with increased antibiotic resistance to cause UTIs.https://www.mdpi.com/2077-0383/8/7/988Uropathogenic E. coliUPECphylogenygenomicsantibiotic resistancevirulence traitskidney transplantation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonas Abo Basha Matthias Kiel Dennis Görlich Katharina Schütte-Nütgen Anika Witten Hermann Pavenstädt Barbara C. Kahl Ulrich Dobrindt Stefan Reuter |
spellingShingle |
Jonas Abo Basha Matthias Kiel Dennis Görlich Katharina Schütte-Nütgen Anika Witten Hermann Pavenstädt Barbara C. Kahl Ulrich Dobrindt Stefan Reuter Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients Journal of Clinical Medicine Uropathogenic E. coli UPEC phylogeny genomics antibiotic resistance virulence traits kidney transplantation |
author_facet |
Jonas Abo Basha Matthias Kiel Dennis Görlich Katharina Schütte-Nütgen Anika Witten Hermann Pavenstädt Barbara C. Kahl Ulrich Dobrindt Stefan Reuter |
author_sort |
Jonas Abo Basha |
title |
Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_short |
Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_full |
Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_fullStr |
Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_full_unstemmed |
Phenotypic and Genotypic Characterization of <i>Escherichia coli</i> Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_sort |
phenotypic and genotypic characterization of <i>escherichia coli</i> causing urinary tract infections in kidney-transplanted patients |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2019-07-01 |
description |
Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed from the UPEC of non-KTx patients. Therefore, we determined the genome sequences of 182 UPEC isolates from KTx and control patients in a large German university clinic and pheno- and genotypically compared these two isolated groups. Resistance to the β-lactams, trimethoprim or trimethoprim/sulfamethoxazole was significantly higher among UPEC from KTx than from control patients, whereas both the isolated groups were highly susceptible to fosfomycin. Accordingly, the gene content conferring resistance to β-lactams or trimethoprim, but also to aminoglycosides, was significantly higher in KTx than in control UPEC isolates. <i>E. coli</i> isolates from KTx patients more frequently presented with uncommon UPEC phylogroups expressing higher numbers of plasmid replicons, but interestingly, less UPEC virulence-associated genes than the control group. We conclude that there is no defining subset of virulence traits for UPEC from KTx patients. The clinical history and immunocompromised status of KTx patients enables <i>E. coli</i> strains with low uropathogenic potential, but with increased antibiotic resistance to cause UTIs. |
topic |
Uropathogenic E. coli UPEC phylogeny genomics antibiotic resistance virulence traits kidney transplantation |
url |
https://www.mdpi.com/2077-0383/8/7/988 |
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