Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition
Abstract Background Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples. However, these associations require further confirmation in the Tunisian olive oil cultivars, and li...
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doaj-94aa4facc33d4be8a797bd7081142ba82020-11-24T21:35:03ZengBMCLipids in Health and Disease1476-511X2017-07-011611610.1186/s12944-017-0530-6Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids compositionRayda Ben Ayed0Karim Ennouri1Hajer Ben Hlima2Slim Smaoui3Mohsen Hanana4Rim Mzid5Sezai Ercişli6Ahmed Rebai7Molecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of SfaxMolecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of SfaxUnité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of SfaxLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of SfaxLaboratory of Extrêmophile Plants, Biotechnology Center of Borj-CédriaLaboratory of Extrêmophile Plants, Biotechnology Center of Borj-CédriaAtaturk University, Faculty of Agriculture, Department of HorticultureMolecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of SfaxAbstract Background Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples. However, these associations require further confirmation in the Tunisian olive oil cultivars, and little is known about the effect of polymorphisms in fatty acid-related genes on olive oil mono- and poly- unsaturated fatty acids distribution. Methods A set of olive oils from 12 Tunisian cultivars was chosen. The fatty acid composition of each olive oil sample was determined by gas chromatography. Statistical and modeling Bayesian analyses were used to assess whether the FAD2.1 and FAD2.3 genotypes were associated with fatty acids composition. Results The TT-FAD2.1 and the GG-FAD2.3 genotypes were found to be associated with a lower proportion of oleic acid (C18:1) (r = −0.778, p = 0.003; r = −0.781, p= 0.003) as well as higher proportion of linoleic (C18:2) (r = 0.693, p = 0.012; r = −0.759, p= 0.004) and palmitic acids (C16:0) (r = 0.643, p = 0.024; r = −0.503, p= 0.095), making varieties with this haplotype (i.e. Chemlali Sfax and Meski) producing more saturated (C16: 0) and polyunsaturated acids than oleic acid. The latter plays a major role in preventing several diseases. Conclusion The two associations FADS2 FAD2.1 and FADS2 FAD2.3 with the fatty acid compositions of olive oil samples were identified among the studied olive cultivars. These associations differed between studied cultivars, which might explain variability in lipidic composition among them and consequently reflecting genetic diversity through differences in gene expression and biochemical pathways. FADS2 locus would constitute thus a good marker for detecting interesting lipidic chemotypes among commercial olive oils.http://link.springer.com/article/10.1186/s12944-017-0530-6Olive oilSNP genotypeQualityFatty acid compositionOleic acidFADS2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rayda Ben Ayed Karim Ennouri Hajer Ben Hlima Slim Smaoui Mohsen Hanana Rim Mzid Sezai Ercişli Ahmed Rebai |
spellingShingle |
Rayda Ben Ayed Karim Ennouri Hajer Ben Hlima Slim Smaoui Mohsen Hanana Rim Mzid Sezai Ercişli Ahmed Rebai Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition Lipids in Health and Disease Olive oil SNP genotype Quality Fatty acid composition Oleic acid FADS2 |
author_facet |
Rayda Ben Ayed Karim Ennouri Hajer Ben Hlima Slim Smaoui Mohsen Hanana Rim Mzid Sezai Ercişli Ahmed Rebai |
author_sort |
Rayda Ben Ayed |
title |
Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition |
title_short |
Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition |
title_full |
Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition |
title_fullStr |
Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition |
title_full_unstemmed |
Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition |
title_sort |
identification and characterization of single nucleotide polymorphism markers in fads2 gene associated with olive oil fatty acids composition |
publisher |
BMC |
series |
Lipids in Health and Disease |
issn |
1476-511X |
publishDate |
2017-07-01 |
description |
Abstract Background Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples. However, these associations require further confirmation in the Tunisian olive oil cultivars, and little is known about the effect of polymorphisms in fatty acid-related genes on olive oil mono- and poly- unsaturated fatty acids distribution. Methods A set of olive oils from 12 Tunisian cultivars was chosen. The fatty acid composition of each olive oil sample was determined by gas chromatography. Statistical and modeling Bayesian analyses were used to assess whether the FAD2.1 and FAD2.3 genotypes were associated with fatty acids composition. Results The TT-FAD2.1 and the GG-FAD2.3 genotypes were found to be associated with a lower proportion of oleic acid (C18:1) (r = −0.778, p = 0.003; r = −0.781, p= 0.003) as well as higher proportion of linoleic (C18:2) (r = 0.693, p = 0.012; r = −0.759, p= 0.004) and palmitic acids (C16:0) (r = 0.643, p = 0.024; r = −0.503, p= 0.095), making varieties with this haplotype (i.e. Chemlali Sfax and Meski) producing more saturated (C16: 0) and polyunsaturated acids than oleic acid. The latter plays a major role in preventing several diseases. Conclusion The two associations FADS2 FAD2.1 and FADS2 FAD2.3 with the fatty acid compositions of olive oil samples were identified among the studied olive cultivars. These associations differed between studied cultivars, which might explain variability in lipidic composition among them and consequently reflecting genetic diversity through differences in gene expression and biochemical pathways. FADS2 locus would constitute thus a good marker for detecting interesting lipidic chemotypes among commercial olive oils. |
topic |
Olive oil SNP genotype Quality Fatty acid composition Oleic acid FADS2 |
url |
http://link.springer.com/article/10.1186/s12944-017-0530-6 |
work_keys_str_mv |
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