Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects
Radix Pseudostellariae, the root of Pseudostellaria heterophylla (Miq.) Pax, has historically been used as medicine food homology plant for thousand years in China. Herein, PHP, a water soluble polysaccharides from Radix Pseudostellariae, was isolated and identified by HPGPC, GC–MS, FT-IR, and scann...
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Format: | Article |
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Elsevier
2021-03-01
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Series: | Journal of Functional Foods |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621000025 |
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doaj-94e956ce436242caa26f0cd7bc0558ab |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Siyuan You Xiawan Liu Guitao Xu Mingzhu Ye Lisha Bai Rongxiao Lin Xinrui Sha Lifang Liang Jun Huang Chang Zhou Wen Rui Hongyuan Chen |
spellingShingle |
Siyuan You Xiawan Liu Guitao Xu Mingzhu Ye Lisha Bai Rongxiao Lin Xinrui Sha Lifang Liang Jun Huang Chang Zhou Wen Rui Hongyuan Chen Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects Journal of Functional Foods Radix Pseudostellaria Polysaccharide Anti-inflammatory Inflammatory bowel disease Intestinal flora |
author_facet |
Siyuan You Xiawan Liu Guitao Xu Mingzhu Ye Lisha Bai Rongxiao Lin Xinrui Sha Lifang Liang Jun Huang Chang Zhou Wen Rui Hongyuan Chen |
author_sort |
Siyuan You |
title |
Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects |
title_short |
Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects |
title_full |
Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects |
title_fullStr |
Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects |
title_full_unstemmed |
Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects |
title_sort |
identification of bioactive polysaccharide from pseudostellaria heterophylla with its anti-inflammatory effects |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2021-03-01 |
description |
Radix Pseudostellariae, the root of Pseudostellaria heterophylla (Miq.) Pax, has historically been used as medicine food homology plant for thousand years in China. Herein, PHP, a water soluble polysaccharides from Radix Pseudostellariae, was isolated and identified by HPGPC, GC–MS, FT-IR, and scanning electron microscopy. Results showed that PHP (Mw = 1.07 × 104 kDa) mainly consisted of rhamnose, galacturonic acid, glucose, galactose, arabinose, fucose in a molar ratio of 0.055:0.208:0.306:0.055:0.753:0.594:0.014. It contained nine types of linkages, including → 6)-Galp(A)-(1→, →6)-Galp-(1→, →3)-Arap-(1→, Galp-(1→, →3)-Rhap-(1→, Glup-(1→, →3)-Araf-(1→, →2,3,4)-Manp-(1 → and → 3,4)-Fucp-(1→. Morphology analysis showed that PHP took flakes with irregular size and properties. It significantly activated TLR4 in vivo. In DSS induced mouse-acute-colitis molds, PHP90-3a improved and the colon length was on ulcerative in mice. the production of IL-1β and TNF-α were decreased. The intestinal mucosa was more complete, there were much more goblet cells, and the infiltration of lymphocytes and neutrophils was significantly decreased. The content of SCFA increased, whereas acetic acid, propionic acid and butyric acid increased. 16S RNA sequencing and OTUs cluster analysis revealed that Bacteroides and Pseudomonas were the dominant intestinal flora. The analysis of community thermogram showed that the abundance ratio of Pseudomonas and Bacteroides (F/B) was significantly lower than the dominant group and significantly inhibited the levels of Verrucomicrobia and Akkermansia. Altogether, the data suggested that the anti-inflammatory activity might be implicated with the activation of TLR4 signal pathways, reducing IL-1β and TNF-α, and the regulation of mucosal immunity and the composition of intestinal flora. |
topic |
Radix Pseudostellaria Polysaccharide Anti-inflammatory Inflammatory bowel disease Intestinal flora |
url |
http://www.sciencedirect.com/science/article/pii/S1756464621000025 |
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doaj-94e956ce436242caa26f0cd7bc0558ab2021-04-30T07:20:27ZengElsevierJournal of Functional Foods1756-46462021-03-0178104353Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effectsSiyuan You0Xiawan Liu1Guitao Xu2Mingzhu Ye3Lisha Bai4Rongxiao Lin5Xinrui Sha6Lifang Liang7Jun Huang8Chang Zhou9Wen Rui10Hongyuan Chen11Department of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaDepartment of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaKey Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaCentrefor Novel Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaCentrefor Novel Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaDepartment of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaDepartment of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaDepartment of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaKey Laboratory of Immunology, Sino-French Hoffmann Institute, School of Basic Medical Sciences; Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, Guangdong Province, PR ChinaDepartment of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR ChinaCentrefor Novel Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China; Corresponding authors at: Department of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China (H. Chen).Department of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China; Guangdong Engineering & Technology Research Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China; Guangdong Cosmetics Engineering & Technology Research Center, Zhongshan 528458, Guangdong Province, PR China; Corresponding authors at: Department of Pathogenic Biology and Immunology, School of Life Sciences and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, PR China (H. Chen).Radix Pseudostellariae, the root of Pseudostellaria heterophylla (Miq.) Pax, has historically been used as medicine food homology plant for thousand years in China. Herein, PHP, a water soluble polysaccharides from Radix Pseudostellariae, was isolated and identified by HPGPC, GC–MS, FT-IR, and scanning electron microscopy. Results showed that PHP (Mw = 1.07 × 104 kDa) mainly consisted of rhamnose, galacturonic acid, glucose, galactose, arabinose, fucose in a molar ratio of 0.055:0.208:0.306:0.055:0.753:0.594:0.014. It contained nine types of linkages, including → 6)-Galp(A)-(1→, →6)-Galp-(1→, →3)-Arap-(1→, Galp-(1→, →3)-Rhap-(1→, Glup-(1→, →3)-Araf-(1→, →2,3,4)-Manp-(1 → and → 3,4)-Fucp-(1→. Morphology analysis showed that PHP took flakes with irregular size and properties. It significantly activated TLR4 in vivo. In DSS induced mouse-acute-colitis molds, PHP90-3a improved and the colon length was on ulcerative in mice. the production of IL-1β and TNF-α were decreased. The intestinal mucosa was more complete, there were much more goblet cells, and the infiltration of lymphocytes and neutrophils was significantly decreased. The content of SCFA increased, whereas acetic acid, propionic acid and butyric acid increased. 16S RNA sequencing and OTUs cluster analysis revealed that Bacteroides and Pseudomonas were the dominant intestinal flora. The analysis of community thermogram showed that the abundance ratio of Pseudomonas and Bacteroides (F/B) was significantly lower than the dominant group and significantly inhibited the levels of Verrucomicrobia and Akkermansia. Altogether, the data suggested that the anti-inflammatory activity might be implicated with the activation of TLR4 signal pathways, reducing IL-1β and TNF-α, and the regulation of mucosal immunity and the composition of intestinal flora.http://www.sciencedirect.com/science/article/pii/S1756464621000025Radix PseudostellariaPolysaccharideAnti-inflammatoryInflammatory bowel diseaseIntestinal flora |