Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes
Tumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, compl...
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doaj-94ed18819b1a494aa1bf8fc5fd6f94cb2020-11-25T00:35:56ZengMDPI AGCancers2072-66942019-05-0111568210.3390/cancers11050682cancers11050682Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph NodesAnna Rachel Young0Jessica Da Gama Duarte1Rhiannon Coulson2Megan O’Brien3Siddhartha Deb4Alex Lopata5Andreas Behren6Suresh Mathivanan7Elgene Lim8Els Meeusen9La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne 3086, AustraliaOlivia Newton-John Cancer Research Institute, Level 5, ONJ Centre, Heidelberg Vic 3084, AustraliaGarvan Institute of Medical Research, St Vincent’s Clinical School, Darlinghurst, NSW 2010, AustraliaOlivia Newton-John Cancer Research Institute, Level 5, ONJ Centre, Heidelberg Vic 3084, AustraliaConsultant Pathologist, Anatpath. 120 Gardenvale Rd, Gardenvale Melbourne 3185, AustraliaCancerProbe Pty Ltd, PO Box 2237, Prahran 3181, AustraliaOlivia Newton-John Cancer Research Institute, Level 5, ONJ Centre, Heidelberg Vic 3084, AustraliaLa Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne 3086, AustraliaGarvan Institute of Medical Research, St Vincent’s Clinical School, Darlinghurst, NSW 2010, AustraliaLa Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne 3086, AustraliaTumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, complex formation, and background reactivity. In this study, antibodies were generated from antibody-secreting cells (ASC) present in tumor-draining lymph nodes of 20 breast cancer patients (ASC-probes) and were used to screen breast cancer cell lines and protein microarrays. Half of the ASC-probes reacted strongly against extracts of the MCF-7 breast cancer cell line, but each with a distinct antigen recognition profile. Three of the positive ASC-probes reacted differentially with recombinant antigens on a microarray containing cancer-related proteins. The results of this study show that lymph node-derived ASC-probes provide a highly specific source of tumor-specific antibodies. Each breast cancer patient reacts with a different antibody profile which indicates that targeted immunotherapies may need to be personalized for individual patients. Focused microarrays in combination with ASC-probes may be useful in providing immune profiles and identifying tumor antigens of individual cancer patients.https://www.mdpi.com/2072-6694/11/5/682tumor antigenimmune profilelymph nodeantibody secreting cellbiomarkermicroarraybreast cancerimmunotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Rachel Young Jessica Da Gama Duarte Rhiannon Coulson Megan O’Brien Siddhartha Deb Alex Lopata Andreas Behren Suresh Mathivanan Elgene Lim Els Meeusen |
spellingShingle |
Anna Rachel Young Jessica Da Gama Duarte Rhiannon Coulson Megan O’Brien Siddhartha Deb Alex Lopata Andreas Behren Suresh Mathivanan Elgene Lim Els Meeusen Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes Cancers tumor antigen immune profile lymph node antibody secreting cell biomarker microarray breast cancer immunotherapy |
author_facet |
Anna Rachel Young Jessica Da Gama Duarte Rhiannon Coulson Megan O’Brien Siddhartha Deb Alex Lopata Andreas Behren Suresh Mathivanan Elgene Lim Els Meeusen |
author_sort |
Anna Rachel Young |
title |
Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes |
title_short |
Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes |
title_full |
Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes |
title_fullStr |
Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes |
title_full_unstemmed |
Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes |
title_sort |
immunoprofiling of breast cancer antigens using antibodies derived from local lymph nodes |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2019-05-01 |
description |
Tumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, complex formation, and background reactivity. In this study, antibodies were generated from antibody-secreting cells (ASC) present in tumor-draining lymph nodes of 20 breast cancer patients (ASC-probes) and were used to screen breast cancer cell lines and protein microarrays. Half of the ASC-probes reacted strongly against extracts of the MCF-7 breast cancer cell line, but each with a distinct antigen recognition profile. Three of the positive ASC-probes reacted differentially with recombinant antigens on a microarray containing cancer-related proteins. The results of this study show that lymph node-derived ASC-probes provide a highly specific source of tumor-specific antibodies. Each breast cancer patient reacts with a different antibody profile which indicates that targeted immunotherapies may need to be personalized for individual patients. Focused microarrays in combination with ASC-probes may be useful in providing immune profiles and identifying tumor antigens of individual cancer patients. |
topic |
tumor antigen immune profile lymph node antibody secreting cell biomarker microarray breast cancer immunotherapy |
url |
https://www.mdpi.com/2072-6694/11/5/682 |
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