H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis
Hydrogen sulfide (H2S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-03-01
|
Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.635470/full |
id |
doaj-94f00ac6b0724afea76ac329ea1fe90f |
---|---|
record_format |
Article |
spelling |
doaj-94f00ac6b0724afea76ac329ea1fe90f2021-03-11T07:33:59ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-03-01810.3389/fmolb.2021.635470635470H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in ArabidopsisXinzhe Du0Xinzhe Du1Zhuping Jin2Zhiqiang Liu3Danmei Liu4Liping Zhang5Xiaoli Ma6Guangdong Yang7Sha Liu8Yarong Guo9Yanxi Pei10Department of Psychiatry, Firtst Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaDepartment of Chemistry and Biochemistry, Laurentian University, Sudbury, ON, CanadaDepartment of Psychiatry, Firtst Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaSchool of Life Science, Shanxi University, Taiyuan, ChinaHydrogen sulfide (H2S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of H2S involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of H2S to alleviate cold stress is impaired in mpk4 mutants, but not in the upstream mek2 and crlk1 mutants. MPK4 was basically persulfidated, and NaHS (H2S donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by H2S. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that H2S alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity.https://www.frontiersin.org/articles/10.3389/fmolb.2021.635470/fullhydrogen sulfidePersulfidationMPK4MEK2cold stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xinzhe Du Xinzhe Du Zhuping Jin Zhiqiang Liu Danmei Liu Liping Zhang Xiaoli Ma Guangdong Yang Sha Liu Yarong Guo Yanxi Pei |
spellingShingle |
Xinzhe Du Xinzhe Du Zhuping Jin Zhiqiang Liu Danmei Liu Liping Zhang Xiaoli Ma Guangdong Yang Sha Liu Yarong Guo Yanxi Pei H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis Frontiers in Molecular Biosciences hydrogen sulfide Persulfidation MPK4 MEK2 cold stress |
author_facet |
Xinzhe Du Xinzhe Du Zhuping Jin Zhiqiang Liu Danmei Liu Liping Zhang Xiaoli Ma Guangdong Yang Sha Liu Yarong Guo Yanxi Pei |
author_sort |
Xinzhe Du |
title |
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis |
title_short |
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis |
title_full |
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis |
title_fullStr |
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis |
title_full_unstemmed |
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis |
title_sort |
h2s persulfidated and increased kinase activity of mpk4 to response cold stress in arabidopsis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Biosciences |
issn |
2296-889X |
publishDate |
2021-03-01 |
description |
Hydrogen sulfide (H2S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of H2S involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of H2S to alleviate cold stress is impaired in mpk4 mutants, but not in the upstream mek2 and crlk1 mutants. MPK4 was basically persulfidated, and NaHS (H2S donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by H2S. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that H2S alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity. |
topic |
hydrogen sulfide Persulfidation MPK4 MEK2 cold stress |
url |
https://www.frontiersin.org/articles/10.3389/fmolb.2021.635470/full |
work_keys_str_mv |
AT xinzhedu h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT xinzhedu h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT zhupingjin h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT zhiqiangliu h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT danmeiliu h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT lipingzhang h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT xiaolima h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT guangdongyang h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT shaliu h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT yarongguo h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis AT yanxipei h2spersulfidatedandincreasedkinaseactivityofmpk4toresponsecoldstressinarabidopsis |
_version_ |
1724225938512674816 |