Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients
Renal transplantation is life-changing in many aspects. This includes changes to the gut microbiome likely due to exposure to immunosuppressive drugs and antibiotics. As a consequence, renal transplant recipients (RTRs) might suffer from intestinal dysbiosis. We aimed to investigate the gut microbio...
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doaj-94f0715e31e949a3b884b090a1283dd32020-11-25T02:05:26ZengMDPI AGJournal of Clinical Medicine2077-03832020-02-019238610.3390/jcm9020386jcm9020386Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant RecipientsJ. Casper Swarte0Rianne M. Douwes1Shixian Hu2Arnau Vich Vila3Michele F. Eisenga4Marco van Londen5António W. Gomes-Neto6Rinse K. Weersma7Hermie J.M. Harmsen8Stephan J.L. Bakker9Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Medical Microbiology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsDepartment of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700RB Groningen, The NetherlandsRenal transplantation is life-changing in many aspects. This includes changes to the gut microbiome likely due to exposure to immunosuppressive drugs and antibiotics. As a consequence, renal transplant recipients (RTRs) might suffer from intestinal dysbiosis. We aimed to investigate the gut microbiome of RTRs and compare it with healthy controls and to identify determinants of the gut microbiome of RTRs. Therefore, RTRs and healthy controls participating in the TransplantLines Biobank and Cohort Study (NCT03272841) were included. We analyzed the gut microbiome using 16S rRNA sequencing and compared the composition of the gut microbiome of RTRs to healthy controls using multivariate association with linear models (MaAsLin). Fecal samples of 139 RTRs (50% male, mean age: 58.3 ± 12.8 years) and 105 healthy controls (57% male, mean age: 59.2 ± 10.6 years) were collected. Median time after transplantation of RTRs was 6.0 (1.5−12.5)years. The microbiome composition of RTRs was significantly different from that of healthy controls, and RTRs had a lower diversity of the gut microbiome (<i>p</i> < 0.01). Proton-pump inhibitors, mycophenolate mofetil, and estimated glomerular filtration rate (eGFR) are significant determinants of the gut microbiome of RTRs (<i>p</i> < 0.05). Use of mycophenolate mofetil correlated to a lower diversity (<i>p</i> < 0.01). Moreover, significant alterations were found in multiple bacterial taxa between RTRs and healthy controls. The gut microbiome of RTRs contained more Proteobacteria and less Actinobacteria, and there was a loss of butyrate-producing bacteria in the gut microbiome of RTRs. By comparing the gut microbiome of RTRs to healthy controls we have shown that RTRs suffer from dysbiosis, a disruption in the balance of the gut microbiome.https://www.mdpi.com/2077-0383/9/2/386gut microbiomerenal transplant recipientdiarrheaimmunosuppressive medicationgut microbiotakidney transplantation16s rrna sequencingbutyrate-producing bacteriaproteobacteria |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J. Casper Swarte Rianne M. Douwes Shixian Hu Arnau Vich Vila Michele F. Eisenga Marco van Londen António W. Gomes-Neto Rinse K. Weersma Hermie J.M. Harmsen Stephan J.L. Bakker |
spellingShingle |
J. Casper Swarte Rianne M. Douwes Shixian Hu Arnau Vich Vila Michele F. Eisenga Marco van Londen António W. Gomes-Neto Rinse K. Weersma Hermie J.M. Harmsen Stephan J.L. Bakker Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients Journal of Clinical Medicine gut microbiome renal transplant recipient diarrhea immunosuppressive medication gut microbiota kidney transplantation 16s rrna sequencing butyrate-producing bacteria proteobacteria |
author_facet |
J. Casper Swarte Rianne M. Douwes Shixian Hu Arnau Vich Vila Michele F. Eisenga Marco van Londen António W. Gomes-Neto Rinse K. Weersma Hermie J.M. Harmsen Stephan J.L. Bakker |
author_sort |
J. Casper Swarte |
title |
Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients |
title_short |
Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients |
title_full |
Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients |
title_fullStr |
Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients |
title_full_unstemmed |
Characteristics and Dysbiosis of the Gut Microbiome in Renal Transplant Recipients |
title_sort |
characteristics and dysbiosis of the gut microbiome in renal transplant recipients |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-02-01 |
description |
Renal transplantation is life-changing in many aspects. This includes changes to the gut microbiome likely due to exposure to immunosuppressive drugs and antibiotics. As a consequence, renal transplant recipients (RTRs) might suffer from intestinal dysbiosis. We aimed to investigate the gut microbiome of RTRs and compare it with healthy controls and to identify determinants of the gut microbiome of RTRs. Therefore, RTRs and healthy controls participating in the TransplantLines Biobank and Cohort Study (NCT03272841) were included. We analyzed the gut microbiome using 16S rRNA sequencing and compared the composition of the gut microbiome of RTRs to healthy controls using multivariate association with linear models (MaAsLin). Fecal samples of 139 RTRs (50% male, mean age: 58.3 ± 12.8 years) and 105 healthy controls (57% male, mean age: 59.2 ± 10.6 years) were collected. Median time after transplantation of RTRs was 6.0 (1.5−12.5)years. The microbiome composition of RTRs was significantly different from that of healthy controls, and RTRs had a lower diversity of the gut microbiome (<i>p</i> < 0.01). Proton-pump inhibitors, mycophenolate mofetil, and estimated glomerular filtration rate (eGFR) are significant determinants of the gut microbiome of RTRs (<i>p</i> < 0.05). Use of mycophenolate mofetil correlated to a lower diversity (<i>p</i> < 0.01). Moreover, significant alterations were found in multiple bacterial taxa between RTRs and healthy controls. The gut microbiome of RTRs contained more Proteobacteria and less Actinobacteria, and there was a loss of butyrate-producing bacteria in the gut microbiome of RTRs. By comparing the gut microbiome of RTRs to healthy controls we have shown that RTRs suffer from dysbiosis, a disruption in the balance of the gut microbiome. |
topic |
gut microbiome renal transplant recipient diarrhea immunosuppressive medication gut microbiota kidney transplantation 16s rrna sequencing butyrate-producing bacteria proteobacteria |
url |
https://www.mdpi.com/2077-0383/9/2/386 |
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