Improved procedure for the extraction of lipids from human erythrocytes

A procedure for the extraction of human erythrocyte lipids using chloroform–isopropanol 7:11 (v/v) is described. It is simple and reproducible, affords almost quantitative extraction of cholesterol and phospholipid, uses a single extraction tube, and yields an extract only slightly contaminated by h...

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Bibliographic Details
Main Authors:	Herbert G. Rose, Morris Oklander
Format:	Article
Language:	English
Published:	Elsevier 1965-07-01
Series:	Journal of Lipid Research
Subjects:	human erythrocyte lipid extraction chloroform-isopropanol Folch
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520393147

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spelling	doaj-951240feeae84f6f8ceaee10a322d79f2021-04-23T06:11:31ZengElsevierJournal of Lipid Research0022-22751965-07-0163428431Improved procedure for the extraction of lipids from human erythrocytesHerbert G. Rose0Morris Oklander1General Medical Research Laboratory, VA Hospital, Bronx, New YorkGeneral Medical Research Laboratory, VA Hospital, Bronx, New YorkA procedure for the extraction of human erythrocyte lipids using chloroform–isopropanol 7:11 (v/v) is described. It is simple and reproducible, affords almost quantitative extraction of cholesterol and phospholipid, uses a single extraction tube, and yields an extract only slightly contaminated by heme.http://www.sciencedirect.com/science/article/pii/S0022227520393147humanerythrocytelipidextractionchloroform-isopropanolFolch
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Herbert G. Rose Morris Oklander
spellingShingle	Herbert G. Rose Morris Oklander Improved procedure for the extraction of lipids from human erythrocytes Journal of Lipid Research human erythrocyte lipid extraction chloroform-isopropanol Folch
author_facet	Herbert G. Rose Morris Oklander
author_sort	Herbert G. Rose
title	Improved procedure for the extraction of lipids from human erythrocytes
title_short	Improved procedure for the extraction of lipids from human erythrocytes
title_full	Improved procedure for the extraction of lipids from human erythrocytes
title_fullStr	Improved procedure for the extraction of lipids from human erythrocytes
title_full_unstemmed	Improved procedure for the extraction of lipids from human erythrocytes
title_sort	improved procedure for the extraction of lipids from human erythrocytes
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1965-07-01
description	A procedure for the extraction of human erythrocyte lipids using chloroform–isopropanol 7:11 (v/v) is described. It is simple and reproducible, affords almost quantitative extraction of cholesterol and phospholipid, uses a single extraction tube, and yields an extract only slightly contaminated by heme.
topic	human erythrocyte lipid extraction chloroform-isopropanol Folch
url	http://www.sciencedirect.com/science/article/pii/S0022227520393147
work_keys_str_mv	AT herbertgrose improvedprocedurefortheextractionoflipidsfromhumanerythrocytes AT morrisoklander improvedprocedurefortheextractionoflipidsfromhumanerythrocytes
_version_	1721513461458927616

Improved procedure for the extraction of lipids from human erythrocytes

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