| Summary: | <i>Background:</i> The linearly increased loading (RAMP) incremental test is a method commonly used to evaluate physical performance in a laboratory, but the best-designed protocol remains unknown. The aim of this study was to compare the selected variables used in training control resulting from the two different intensities of RAMP incremental tests. <i>Methods:</i> Twenty healthy and physically active men took part in this experiment. The tests included two visits to a laboratory, during which anthropometric measurements, incremental test on a cycle ergometer, and examinations of heart rate and blood lactate concentration were made. The cross-over study design method was used. The subjects underwent a randomly selected RAMP test with incremental load: 0.278 W·s<sup>−1</sup> or 0.556 W·s<sup>−1</sup>. They performed the second test a week later. <i>Results:</i> Peak power output was significantly higher by 51.69 W (<i>p</i> < 0.001; t = 13.10; ES = 1.13) in the 0.556 W·s<sup>−1</sup> group. Total work done was significantly higher in the 0.278 W·s<sup>−1</sup> group by 71.93 kJ (<i>p</i> < 0.001; t = 12.55; ES = 1.57). Maximal heart rate was significantly higher in the 0.278 W·s<sup>−1</sup> group by 3.30 bpm (<i>p</i> < 0.01; t = 3.72; ES = 0.48). There were no statistically significant differences in heart rate recovery and peak blood lactate. <i>Conclusions:</i> We recommend use of the 0.556 W·s<sup>−1</sup> RAMP protocol because it is of shorter duration compared with 0.278 W·s<sup>−1</sup> and as such practically easier and of less effort for subjects.
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