Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry
Early diagnosis of colorectal cancer is crucial to increase the survival rates of the patients and breath analysis represents a promising non-invasive tool to obtain information on cancer-related variations on the human volatilome. A solid phase microextraction coupled to gas chromatography–mass spe...
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doaj-95335e6248b344edb5c3672f044b69642021-08-06T15:19:07ZengMDPI AGApplied Sciences2076-34172021-07-01116910691010.3390/app11156910Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass SpectrometryNicoletta De Vietro0Antonella Maria Aresta1Arcangelo Picciariello2Maria Teresa Rotelli3Carlo Zambonin4Department of Chemistry, University “Aldo Moro”, Via Orabona, 4, 70126 Bari, ItalyDepartment of Chemistry, University “Aldo Moro”, Via Orabona, 4, 70126 Bari, ItalySurgical Unit “M. Rubino”, Department of Emergency and Organ Transplantation, University “Aldo Moro”, 70126 Bari, ItalySurgical Unit “M. Rubino”, Department of Emergency and Organ Transplantation, University “Aldo Moro”, 70126 Bari, ItalyDepartment of Chemistry, University “Aldo Moro”, Via Orabona, 4, 70126 Bari, ItalyEarly diagnosis of colorectal cancer is crucial to increase the survival rates of the patients and breath analysis represents a promising non-invasive tool to obtain information on cancer-related variations on the human volatilome. A solid phase microextraction coupled to gas chromatography–mass spectrometry method for the determination of seven selected compounds, representative of the volatilome secreted by the colonic mucosa of patients affected by colorectal cancer, including benzaldehyde, benzoic acid, dodecane, ethylbenzene, octanal, tetradecane and toluene, was developed. All the extraction parameters were studied for both headspace and direct immersion sampling and the procedures fully validated. The potential of the approach was demonstrated by the time monitoring of the emission of the selected volatile organic compounds from the surgical resected colon mucosa tissues of colorectal cancer patients. Furthermore, the extraction and identification of thirty-one volatile organic compounds secreted by the same tissues was accomplished.https://www.mdpi.com/2076-3417/11/15/6910headspace solid phase microextractiondirect immersion solid phase microextractiongas chromatography–mass spectrometryvolatile organic compoundssurgical resected tissuescolorectal cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicoletta De Vietro Antonella Maria Aresta Arcangelo Picciariello Maria Teresa Rotelli Carlo Zambonin |
spellingShingle |
Nicoletta De Vietro Antonella Maria Aresta Arcangelo Picciariello Maria Teresa Rotelli Carlo Zambonin Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry Applied Sciences headspace solid phase microextraction direct immersion solid phase microextraction gas chromatography–mass spectrometry volatile organic compounds surgical resected tissues colorectal cancer |
author_facet |
Nicoletta De Vietro Antonella Maria Aresta Arcangelo Picciariello Maria Teresa Rotelli Carlo Zambonin |
author_sort |
Nicoletta De Vietro |
title |
Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry |
title_short |
Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry |
title_full |
Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry |
title_fullStr |
Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry |
title_full_unstemmed |
Determination of VOCs in Surgical Resected Tissues from Colorectal Cancer Patients by Solid Phase Microextraction Coupled to Gas Chromatography–Mass Spectrometry |
title_sort |
determination of vocs in surgical resected tissues from colorectal cancer patients by solid phase microextraction coupled to gas chromatography–mass spectrometry |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-07-01 |
description |
Early diagnosis of colorectal cancer is crucial to increase the survival rates of the patients and breath analysis represents a promising non-invasive tool to obtain information on cancer-related variations on the human volatilome. A solid phase microextraction coupled to gas chromatography–mass spectrometry method for the determination of seven selected compounds, representative of the volatilome secreted by the colonic mucosa of patients affected by colorectal cancer, including benzaldehyde, benzoic acid, dodecane, ethylbenzene, octanal, tetradecane and toluene, was developed. All the extraction parameters were studied for both headspace and direct immersion sampling and the procedures fully validated. The potential of the approach was demonstrated by the time monitoring of the emission of the selected volatile organic compounds from the surgical resected colon mucosa tissues of colorectal cancer patients. Furthermore, the extraction and identification of thirty-one volatile organic compounds secreted by the same tissues was accomplished. |
topic |
headspace solid phase microextraction direct immersion solid phase microextraction gas chromatography–mass spectrometry volatile organic compounds surgical resected tissues colorectal cancer |
url |
https://www.mdpi.com/2076-3417/11/15/6910 |
work_keys_str_mv |
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