Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli

Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli

Human BiP/GRP78 is involved in the folding and assembly of proteins in the endoplasmic reticulum. The proteins for crystallization in good amount and quality are prerequisites for obtaining ideal crystals. To meet these requirements, different BiP/GRP78 constructs, competent cells, vectors, and conc...

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Bibliographic Details
Main Authors: Jiao Yang, Lei Zhou, Qinglian Liu
Format: Article
Language:English
Published: Elsevier 2017-02-01
Series:Data in Brief
Subjects:
Hsp70
BiP/GRP78
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916305054
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spelling doaj-953ac01a99a94096a9f8d5417c8b7e002020-11-25T01:44:10ZengElsevierData in Brief2352-34092017-02-0110C52553010.1016/j.dib.2016.08.006Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coliJiao YangLei ZhouQinglian LiuHuman BiP/GRP78 is involved in the folding and assembly of proteins in the endoplasmic reticulum. The proteins for crystallization in good amount and quality are prerequisites for obtaining ideal crystals. To meet these requirements, different BiP/GRP78 constructs, competent cells, vectors, and concentrations of inducer were tested in order to obtain soluble BiP/GRP78 protein with the highest amount and best purity. The BiP–T229A–L3,4′–Smt3 fusion protein was expressed in a soluble manner and finally purified with the highest purity using size exclusion chromatography, which was suitable for further protein crystallization.http://www.sciencedirect.com/science/article/pii/S2352340916305054Hsp70BiP/GRP78Protein expression and purification
collection DOAJ
language English
format Article
sources DOAJ
author Jiao Yang
Lei Zhou
Qinglian Liu
spellingShingle Jiao Yang
Lei Zhou
Qinglian Liu
Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
Data in Brief
Hsp70
BiP/GRP78
Protein expression and purification
author_facet Jiao Yang
Lei Zhou
Qinglian Liu
author_sort Jiao Yang
title Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
title_short Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
title_full Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
title_fullStr Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
title_full_unstemmed Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
title_sort data on the optimizations of expression and purification of human bip/grp78 protein in escherichia coli
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2017-02-01
description Human BiP/GRP78 is involved in the folding and assembly of proteins in the endoplasmic reticulum. The proteins for crystallization in good amount and quality are prerequisites for obtaining ideal crystals. To meet these requirements, different BiP/GRP78 constructs, competent cells, vectors, and concentrations of inducer were tested in order to obtain soluble BiP/GRP78 protein with the highest amount and best purity. The BiP–T229A–L3,4′–Smt3 fusion protein was expressed in a soluble manner and finally purified with the highest purity using size exclusion chromatography, which was suitable for further protein crystallization.
topic Hsp70
BiP/GRP78
Protein expression and purification
url http://www.sciencedirect.com/science/article/pii/S2352340916305054
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AT qinglianliu dataontheoptimizationsofexpressionandpurificationofhumanbipgrp78proteininescherichiacoli
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