Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
Ning Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People&...
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doaj-953b4a5c5b0445b49a86c06fe37ca2c72020-11-25T03:40:51ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity : Targets and Therapy1178-70072020-10-01Volume 134041404958588Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction ModelZhu NYan XLi HWang HNing Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People’s Republic of ChinaTel +86-312-5096409Email zhuning_my@21cn.comBackground: In this study, we explored the clinical significance of serum peroxisome proliferator-activated receptor gamma co-activator 1 (PGC-1) alpha levels in diabetes mellitus with myocardial infarction (DMMI) patients and investigated the possible mechanism.Materials and Methods: Serum samples were obtained from patients with DMMI or normal volunteer in Baoding First Center Hospital. C57BL/6 mice were induced by a single intraperitoneal (i.p.) injection of 100 mg/kg STZ (streptozocin) for in vivo model. Human myocardial cell lines H9C2 cells were induced with high glucose medium (33 mmol/L glucose) for in vitro model. Western blot was used to analyze the protein expressions in this study.Results: Serum PGC-1 alpha levels were down-regulated in patients with DMMI. There was negative correlation between serum PGC-1 alpha levels and glycated hemoglobin, blood glucose or glucagon in DMMI patients. Recombination of PGC-1 alpha protein decreased the levels of glycated hemoglobin, blood glucose and glucagon, and inhibited oxidative stress and myocardial damage in mice of DMMI. Over-expression of PGC-1 alpha reduced reactive oxygen species (ROS)-oxidative stress, while down-regulation of PGC-1 alpha promoted ROS-oxidative stress via regulation of hemeoxygenase− 1 (HO-1) expression in in vitro model of DMMI. The inhibition of HO-1 expression attenuated the anti-oxidation effects of PGC-1 alpha in vitro.Conclusion: PGC-1 alpha attenuated ROS-oxidative stress in diabetic cardiomyopathy model, and PGC-1 alpha served as a potential intervention to alleviate DMMI in clinical applications.Keywords: PGC-1 alpha, ROS, oxidative stress, diabetes mellitus with myocardial infarctionhttps://www.dovepress.com/clinical-significance-of-serum-pgc-1-alpha-levels-in-diabetes-mellitus-peer-reviewed-article-DMSOpgc-1 alpharosoxidative stressdiabetes mellitus with myocardial infarction |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhu N Yan X Li H Wang H |
spellingShingle |
Zhu N Yan X Li H Wang H Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy pgc-1 alpha ros oxidative stress diabetes mellitus with myocardial infarction |
author_facet |
Zhu N Yan X Li H Wang H |
author_sort |
Zhu N |
title |
Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model |
title_short |
Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model |
title_full |
Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model |
title_fullStr |
Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model |
title_full_unstemmed |
Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model |
title_sort |
clinical significance of serum pgc-1 alpha levels in diabetes mellitus with myocardial infarction patients and reduced ros-oxidative stress in diabetes mellitus with myocardial infarction model |
publisher |
Dove Medical Press |
series |
Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy |
issn |
1178-7007 |
publishDate |
2020-10-01 |
description |
Ning Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People’s Republic of ChinaTel +86-312-5096409Email zhuning_my@21cn.comBackground: In this study, we explored the clinical significance of serum peroxisome proliferator-activated receptor gamma co-activator 1 (PGC-1) alpha levels in diabetes mellitus with myocardial infarction (DMMI) patients and investigated the possible mechanism.Materials and Methods: Serum samples were obtained from patients with DMMI or normal volunteer in Baoding First Center Hospital. C57BL/6 mice were induced by a single intraperitoneal (i.p.) injection of 100 mg/kg STZ (streptozocin) for in vivo model. Human myocardial cell lines H9C2 cells were induced with high glucose medium (33 mmol/L glucose) for in vitro model. Western blot was used to analyze the protein expressions in this study.Results: Serum PGC-1 alpha levels were down-regulated in patients with DMMI. There was negative correlation between serum PGC-1 alpha levels and glycated hemoglobin, blood glucose or glucagon in DMMI patients. Recombination of PGC-1 alpha protein decreased the levels of glycated hemoglobin, blood glucose and glucagon, and inhibited oxidative stress and myocardial damage in mice of DMMI. Over-expression of PGC-1 alpha reduced reactive oxygen species (ROS)-oxidative stress, while down-regulation of PGC-1 alpha promoted ROS-oxidative stress via regulation of hemeoxygenase− 1 (HO-1) expression in in vitro model of DMMI. The inhibition of HO-1 expression attenuated the anti-oxidation effects of PGC-1 alpha in vitro.Conclusion: PGC-1 alpha attenuated ROS-oxidative stress in diabetic cardiomyopathy model, and PGC-1 alpha served as a potential intervention to alleviate DMMI in clinical applications.Keywords: PGC-1 alpha, ROS, oxidative stress, diabetes mellitus with myocardial infarction |
topic |
pgc-1 alpha ros oxidative stress diabetes mellitus with myocardial infarction |
url |
https://www.dovepress.com/clinical-significance-of-serum-pgc-1-alpha-levels-in-diabetes-mellitus-peer-reviewed-article-DMSO |
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