Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains
Objective of this work was to determine the long-term preservation of Yersinia pestis DNA, detected by polymerase chain reaction (PCR) in the bone tissue of rodents, and to quantify content changes over time under the influence of biotic and abiotic factors. Materials and methods. After death of mod...
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2016-12-01
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doaj-9547ed889fb34ecc9404d8484db8b3312021-07-22T07:51:39ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2016-12-0104858710.21055/0370-1069-2016-4-85-87354Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone RemainsV. V. Sutyagin0T. V. Mekka-Mechenko1O. V. Kogay2A. T. Berdibekov3”Taldykorgan Plague Control Station”, TaldykorganM.Aykimbaev Kazakh Scientific Center for Quarantine and Zoonotic Infections, Almaty”Taldykorgan Plague Control Station”, Taldykorgan”Taldykorgan Plague Control Station”, TaldykorganObjective of this work was to determine the long-term preservation of Yersinia pestis DNA, detected by polymerase chain reaction (PCR) in the bone tissue of rodents, and to quantify content changes over time under the influence of biotic and abiotic factors. Materials and methods. After death of model animals infected with virulent Y. pestis strain, their skeletons were freed from soft tissues and placed in the environments where conditions were close to the natural ones. The study of bone remains for the presence of Y. pestis DNA fragments was held immediately after the death of the animals, and then, twice a year. Results and conclusions. The data obtained allow for dividing all the fossil remains of the fallen from plague infection mammals into three separate groups: with high, medium, and low content of bacteria. Further study demonstrates that reduction of Y. pestis DNA concentration in bone tissue of animals after six months of storage does not occur. After a year, a sharp decrease (100-fold) of observable material dilutions for which the identification of detected plasmids is still possible, is registered. After one and a half year of observation, the genes of plague microbe in the bones of experimental animals could not be found. In order to increase the information content of the study of this type of material put forward has been proposal to reestablish the system of observation sites to collect castings and bone remains of mammals.https://journal.microbe.ru/jour/article/view/354yersinia pestispcrdnaplasmid genesbone remainscastings |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
V. V. Sutyagin T. V. Mekka-Mechenko O. V. Kogay A. T. Berdibekov |
spellingShingle |
V. V. Sutyagin T. V. Mekka-Mechenko O. V. Kogay A. T. Berdibekov Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains Проблемы особо опасных инфекций yersinia pestis pcr dna plasmid genes bone remains castings |
author_facet |
V. V. Sutyagin T. V. Mekka-Mechenko O. V. Kogay A. T. Berdibekov |
author_sort |
V. V. Sutyagin |
title |
Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains |
title_short |
Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains |
title_full |
Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains |
title_fullStr |
Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains |
title_full_unstemmed |
Duration of Conservation of Plague Microbe (Yersinia pestis) Genes in Mammalian Bone Remains |
title_sort |
duration of conservation of plague microbe (yersinia pestis) genes in mammalian bone remains |
publisher |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
series |
Проблемы особо опасных инфекций |
issn |
0370-1069 2658-719X |
publishDate |
2016-12-01 |
description |
Objective of this work was to determine the long-term preservation of Yersinia pestis DNA, detected by polymerase chain reaction (PCR) in the bone tissue of rodents, and to quantify content changes over time under the influence of biotic and abiotic factors. Materials and methods. After death of model animals infected with virulent Y. pestis strain, their skeletons were freed from soft tissues and placed in the environments where conditions were close to the natural ones. The study of bone remains for the presence of Y. pestis DNA fragments was held immediately after the death of the animals, and then, twice a year. Results and conclusions. The data obtained allow for dividing all the fossil remains of the fallen from plague infection mammals into three separate groups: with high, medium, and low content of bacteria. Further study demonstrates that reduction of Y. pestis DNA concentration in bone tissue of animals after six months of storage does not occur. After a year, a sharp decrease (100-fold) of observable material dilutions for which the identification of detected plasmids is still possible, is registered. After one and a half year of observation, the genes of plague microbe in the bones of experimental animals could not be found. In order to increase the information content of the study of this type of material put forward has been proposal to reestablish the system of observation sites to collect castings and bone remains of mammals. |
topic |
yersinia pestis pcr dna plasmid genes bone remains castings |
url |
https://journal.microbe.ru/jour/article/view/354 |
work_keys_str_mv |
AT vvsutyagin durationofconservationofplaguemicrobeyersiniapestisgenesinmammalianboneremains AT tvmekkamechenko durationofconservationofplaguemicrobeyersiniapestisgenesinmammalianboneremains AT ovkogay durationofconservationofplaguemicrobeyersiniapestisgenesinmammalianboneremains AT atberdibekov durationofconservationofplaguemicrobeyersiniapestisgenesinmammalianboneremains |
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