Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain
Rubbery taproot disease of sugar beet (RTD), associated with ‘<i>Candidatus</i> Phytoplasma solani’, appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed...
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doaj-9551d9f5cb604e2590e1acf1718e450e2021-09-26T00:44:13ZengMDPI AGMicroorganisms2076-26072021-09-0191950195010.3390/microorganisms9091950Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian PlainŽivko Ćurčić0Andrea Kosovac1Jelena Stepanović2Emil Rekanović3Michael Kube4Bojan Duduk5Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaInstitute of Pesticides and Environmental Protection, 11080 Belgrade, SerbiaInstitute of Pesticides and Environmental Protection, 11080 Belgrade, SerbiaInstitute of Pesticides and Environmental Protection, 11080 Belgrade, SerbiaDepartment of Integrative Infection Biology Crops-Livestock, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Pesticides and Environmental Protection, 11080 Belgrade, SerbiaRubbery taproot disease of sugar beet (RTD), associated with ‘<i>Candidatus</i> Phytoplasma solani’, appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed, symptomatic sugar beets were analysed for phytoplasma infection. Additionally, multilocus sequence analyses of ‘<i>Ca</i>. P. solani’ strains on epidemiologically informative marker genes (<i>tuf</i>, <i>stamp</i> and <i>vmp1</i>) were performed. Symptomatic sugar beets from other countries of the Pannonian Plain (Croatia, Hungary and Austria), one sample from Germany, and red beets from Serbia were included in the analyses. ‘<i>Ca</i>. P. solani’ was detected in sugar beet in all assessed countries, as well as in red beet. Molecular analyses revealed the high genetic variability of ‘<i>Ca</i>. P. solani’ with the presence of all four <i>tuf-</i>types (a, b1, b2 and d), 14 <i>stamp</i> genotypes (seven new) and five <i>vmp1</i> profiles (one new). The most common multilocus genotype in Serbia, Slovakia, Croatia, and Hungary was dSTOLg (tuf-d/STOL/V2-TA). It was dominant on sites with epidemic RTD outbreaks in the Pannonian Plain and in several sugar beet fields with non-epidemic RTD occurrence suggesting the prevalence of a particular epidemiological pathway during the epidemic’s phases.https://www.mdpi.com/2076-2607/9/9/1950sugar beetred beetstolbur phytoplasma‘<i>Candidatus</i> Phytoplasma solani’RTDSBR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Živko Ćurčić Andrea Kosovac Jelena Stepanović Emil Rekanović Michael Kube Bojan Duduk |
spellingShingle |
Živko Ćurčić Andrea Kosovac Jelena Stepanović Emil Rekanović Michael Kube Bojan Duduk Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain Microorganisms sugar beet red beet stolbur phytoplasma ‘<i>Candidatus</i> Phytoplasma solani’ RTD SBR |
author_facet |
Živko Ćurčić Andrea Kosovac Jelena Stepanović Emil Rekanović Michael Kube Bojan Duduk |
author_sort |
Živko Ćurčić |
title |
Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain |
title_short |
Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain |
title_full |
Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain |
title_fullStr |
Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain |
title_full_unstemmed |
Multilocus Genotyping of ‘<i>Candidatus</i> Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain |
title_sort |
multilocus genotyping of ‘<i>candidatus</i> phytoplasma solani’ associated with rubbery taproot disease of sugar beet in the pannonian plain |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-09-01 |
description |
Rubbery taproot disease of sugar beet (RTD), associated with ‘<i>Candidatus</i> Phytoplasma solani’, appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed, symptomatic sugar beets were analysed for phytoplasma infection. Additionally, multilocus sequence analyses of ‘<i>Ca</i>. P. solani’ strains on epidemiologically informative marker genes (<i>tuf</i>, <i>stamp</i> and <i>vmp1</i>) were performed. Symptomatic sugar beets from other countries of the Pannonian Plain (Croatia, Hungary and Austria), one sample from Germany, and red beets from Serbia were included in the analyses. ‘<i>Ca</i>. P. solani’ was detected in sugar beet in all assessed countries, as well as in red beet. Molecular analyses revealed the high genetic variability of ‘<i>Ca</i>. P. solani’ with the presence of all four <i>tuf-</i>types (a, b1, b2 and d), 14 <i>stamp</i> genotypes (seven new) and five <i>vmp1</i> profiles (one new). The most common multilocus genotype in Serbia, Slovakia, Croatia, and Hungary was dSTOLg (tuf-d/STOL/V2-TA). It was dominant on sites with epidemic RTD outbreaks in the Pannonian Plain and in several sugar beet fields with non-epidemic RTD occurrence suggesting the prevalence of a particular epidemiological pathway during the epidemic’s phases. |
topic |
sugar beet red beet stolbur phytoplasma ‘<i>Candidatus</i> Phytoplasma solani’ RTD SBR |
url |
https://www.mdpi.com/2076-2607/9/9/1950 |
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