A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer
We have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a hou...
Main Authors: | Ignacio Juarez, Juan Francisco Toro-Fernandez, Christian Vaquero-Yuste, Marta Molina-Alejandre, Inmaculada Lasa, Remedios Gomez, Adela Lopez, Jose Manuel Martin-Villa, Alberto Gutierrez |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
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Series: | Biology |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-7737/10/6/516 |
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