Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
Abstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been sugg...
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2017-06-01
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doaj-9565494a7e254c2098c2868b24f37f7c2020-12-08T01:41:35ZengNature Publishing GroupScientific Reports2045-23222017-06-017111210.1038/s41598-017-04121-wFicolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivoNinette Genster0Olga Østrup1Camilla Schjalm2Tom Eirik Mollnes3Jack B. Cowland4Peter Garred5Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of CopenhagenCenter for Genomic Medicine, Rigshospitalet, Copenhagen University HospitalDepartment of Immunology, Oslo University Hospital, RikshospitaletDepartment of Immunology, Oslo University Hospital, RikshospitaletThe Granulocyte Research Laboratory, Department of Hematology, Copenhagen University HospitalLaboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of CopenhagenAbstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation.https://doi.org/10.1038/s41598-017-04121-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred |
spellingShingle |
Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo Scientific Reports |
author_facet |
Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred |
author_sort |
Ninette Genster |
title |
Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
title_short |
Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
title_full |
Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
title_fullStr |
Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
title_full_unstemmed |
Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
title_sort |
ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation. |
url |
https://doi.org/10.1038/s41598-017-04121-w |
work_keys_str_mv |
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