Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage
Freezing stress is an important abiotic stress that limiting the yield and the spatial distribution of many important crops. This study was undertaken to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.) along with four canola (Brassica napus) cultivars (Hyola 401, Lord...
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Serbian Genetics Society
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doaj-956c68a8910946f08a69e94bc710ef6a2020-11-25T00:26:42ZengSerbian Genetics SocietyGenetika0534-00121820-60692017-01-0149117318110.2298/GENSR1701173S0534-00121701173SScreening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stageSoorni Jahad0Kazemitabar Seyed Kamal1Kahrizi Danial2Dehestani Ali3Bagheri Nadali4Sari Agricultural Sciences and Natural Resources University (SANRU), Department of Plant Breeding and Biotechnology, Sari, Mazandaran, IranSari Agricultural Sciences and Natural Resources University (SANRU), Department of Plant Breeding and Biotechnology, Sari, Mazandaran, IranRazi University, Department of Agronomy and Plant Breeding, Kermanshah, IranSari Agricultural Sciences and Natural Resources University, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari, IranSari Agricultural Sciences and Natural Resources University (SANRU), Department of Plant Breeding and Biotechnology, Sari, Mazandaran, IranFreezing stress is an important abiotic stress that limiting the yield and the spatial distribution of many important crops. This study was undertaken to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.) along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and Cascade) as experimental control under freezing stress conditions (-14°C for 6 h) to identify lines with high or low level of tolerance to freezing stress for further studies. First, a protocol was developed for large scale screening of camelina germplasm under freezing stress conditions. For this purpose, an experiment with different freezing temperatures (-5, -10, -15 and -20°C) was conducted to find an appropriate temperature that discriminated best between genotypes (i.e. the LT50 temperature). The LT50 values for camelina lines were varied between -10.2 and -17.1°C with an average of -13.94°C for all of the camelina lines. Therefore, we selected the -14°C exposure for 6 h as an appropriate temperature to screening of camelina lines. The principal components of measured parameters (LT50, survival percentage, relative conductivity and scoring) was using principal component analysis that determine freezing-tolerant and freezing-sensitive lines. Among 136 doubled haploid lines, some lines (58, 62 and 101) had higher level of freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing sensitive. The selected lines in a preliminary freezing screening are useful for further evaluations.http://www.doiserbia.nb.rs/img/doi/0534-0012/2017/0534-00121701173S.pdfbiplotcanolaLT50principal componentrelative conductivity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Soorni Jahad Kazemitabar Seyed Kamal Kahrizi Danial Dehestani Ali Bagheri Nadali |
spellingShingle |
Soorni Jahad Kazemitabar Seyed Kamal Kahrizi Danial Dehestani Ali Bagheri Nadali Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage Genetika biplot canola LT50 principal component relative conductivity |
author_facet |
Soorni Jahad Kazemitabar Seyed Kamal Kahrizi Danial Dehestani Ali Bagheri Nadali |
author_sort |
Soorni Jahad |
title |
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage |
title_short |
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage |
title_full |
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage |
title_fullStr |
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage |
title_full_unstemmed |
Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage |
title_sort |
screening of camelina (camelina sativa l.) doubled haploid lines for freezing tolerance in the seedling stage |
publisher |
Serbian Genetics Society |
series |
Genetika |
issn |
0534-0012 1820-6069 |
publishDate |
2017-01-01 |
description |
Freezing stress is an important abiotic stress that limiting the yield and
the spatial distribution of many important crops. This study was undertaken
to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.)
along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and
Cascade) as experimental control under freezing stress conditions (-14°C for
6 h) to identify lines with high or low level of tolerance to freezing stress
for further studies. First, a protocol was developed for large scale
screening of camelina germplasm under freezing stress conditions. For this
purpose, an experiment with different freezing temperatures (-5, -10, -15 and
-20°C) was conducted to find an appropriate temperature that discriminated
best between genotypes (i.e. the LT50 temperature). The LT50 values for
camelina lines were varied between -10.2 and -17.1°C with an average of
-13.94°C for all of the camelina lines. Therefore, we selected the -14°C
exposure for 6 h as an appropriate temperature to screening of camelina
lines. The principal components of measured parameters (LT50, survival
percentage, relative conductivity and scoring) was using principal component
analysis that determine freezing-tolerant and freezing-sensitive lines. Among
136 doubled haploid lines, some lines (58, 62 and 101) had higher level of
freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing
sensitive. The selected lines in a preliminary freezing screening are useful
for further evaluations. |
topic |
biplot canola LT50 principal component relative conductivity |
url |
http://www.doiserbia.nb.rs/img/doi/0534-0012/2017/0534-00121701173S.pdf |
work_keys_str_mv |
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