A helium burst biolistic device adapted to penetrate fragile insect tissues
To compensate for the extremely low penetration efficiency of the original PDS/1000-He Bio Rad biolisticreg; device and the deleterious blast effect, design modifications have been made to the launching module. These modifications were evaluated on Bombyx mori embryos and fragile tissues, such as oo...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Oxford University Press
2001-10-01
|
Series: | Journal of Insect Science |
Subjects: | |
Online Access: | http://www.insectscience.org/1.9/ |
id |
doaj-9572fef708a147babb78aaca1165a6c9 |
---|---|
record_format |
Article |
spelling |
doaj-9572fef708a147babb78aaca1165a6c92020-11-24T22:15:10ZengOxford University PressJournal of Insect Science1536-24422001-10-0119A helium burst biolistic device adapted to penetrate fragile insect tissuesJean-Luc ThomasJérôme BardouSebastien L'hosteBernard MauchampGérard ChavancyTo compensate for the extremely low penetration efficiency of the original PDS/1000-He Bio Rad biolisticreg; device and the deleterious blast effect, design modifications have been made to the launching module. These modifications were evaluated on Bombyx mori embryos and fragile tissues, such as oocytes and imaginal wing disks. The original floppy macrocarrier was replaced by a rigid macrocarrier to avoid the effects of the helium blast. The efficiency of the gene gun bombardment was reinforced by the addition of a focusing nozzle. The reduced blast effect allowed us to carry out high-pressure shootings to small organs with improved penetration. This system allowed potentially all the internal embryonic tissues to be transfected with optimal survival rates. The new module was effective on tissues that are difficult to transfect, such as the epithelial wing disk that is covered by a peripodial membrane, and the ovarian follicle cells that lie under the ovariole cell membrane. The new macrocarrier allowed both an aqueous delivery of particles and an ethanolic dry delivery. No significant differences were noted between these two modes of delivery. The major improvement is the possibility of high pressure shooting correlated with appreciable penetration and a weak blast effect.http://www.insectscience.org/1.9/Biolisticsgene gunBombyx moriinsecttransfectiongene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jean-Luc Thomas Jérôme Bardou Sebastien L'hoste Bernard Mauchamp Gérard Chavancy |
spellingShingle |
Jean-Luc Thomas Jérôme Bardou Sebastien L'hoste Bernard Mauchamp Gérard Chavancy A helium burst biolistic device adapted to penetrate fragile insect tissues Journal of Insect Science Biolistics gene gun Bombyx mori insect transfection gene expression |
author_facet |
Jean-Luc Thomas Jérôme Bardou Sebastien L'hoste Bernard Mauchamp Gérard Chavancy |
author_sort |
Jean-Luc Thomas |
title |
A helium burst biolistic device adapted to penetrate fragile insect tissues |
title_short |
A helium burst biolistic device adapted to penetrate fragile insect tissues |
title_full |
A helium burst biolistic device adapted to penetrate fragile insect tissues |
title_fullStr |
A helium burst biolistic device adapted to penetrate fragile insect tissues |
title_full_unstemmed |
A helium burst biolistic device adapted to penetrate fragile insect tissues |
title_sort |
helium burst biolistic device adapted to penetrate fragile insect tissues |
publisher |
Oxford University Press |
series |
Journal of Insect Science |
issn |
1536-2442 |
publishDate |
2001-10-01 |
description |
To compensate for the extremely low penetration efficiency of the original PDS/1000-He Bio Rad biolisticreg; device and the deleterious blast effect, design modifications have been made to the launching module. These modifications were evaluated on Bombyx mori embryos and fragile tissues, such as oocytes and imaginal wing disks. The original floppy macrocarrier was replaced by a rigid macrocarrier to avoid the effects of the helium blast. The efficiency of the gene gun bombardment was reinforced by the addition of a focusing nozzle. The reduced blast effect allowed us to carry out high-pressure shootings to small organs with improved penetration. This system allowed potentially all the internal embryonic tissues to be transfected with optimal survival rates. The new module was effective on tissues that are difficult to transfect, such as the epithelial wing disk that is covered by a peripodial membrane, and the ovarian follicle cells that lie under the ovariole cell membrane. The new macrocarrier allowed both an aqueous delivery of particles and an ethanolic dry delivery. No significant differences were noted between these two modes of delivery. The major improvement is the possibility of high pressure shooting correlated with appreciable penetration and a weak blast effect. |
topic |
Biolistics gene gun Bombyx mori insect transfection gene expression |
url |
http://www.insectscience.org/1.9/ |
work_keys_str_mv |
AT jeanlucthomas aheliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT jeromebardou aheliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT sebastienlhoste aheliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT bernardmauchamp aheliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT gerardchavancy aheliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT jeanlucthomas heliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT jeromebardou heliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT sebastienlhoste heliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT bernardmauchamp heliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues AT gerardchavancy heliumburstbiolisticdeviceadaptedtopenetratefragileinsecttissues |
_version_ |
1725795690222714880 |