Effects of different components of Mao Dongqing’s total flavonoids and total saponins on transient ischemic attack (TIA) model of rats

• Main Authors: Ming-San Miao, Meng-fan Peng, Rui-juan Ma, Ming Bai, Bao-song Liu
• Format: Article
• Language: English
• Published: Elsevier 2018-03-01
• Series: Saudi Journal of Biological Sciences
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X17302619

Objective: To study the effects of the different components of the total flavonoids and total saponins from Mao Dongqing's active site on the rats of TIA model, determine the optimal reactive components ratio of Mao Dongqing on the rats of TIA. Methods: TIA rat model was induced by tail vein injection of tert butyl alcohol, the blank group was injected with the same amount of physiological saline, then behavioral score wasevaluated.Determination the level of glutamic acid in serum, the activity of Na+-K+-ATP enzyme, CA++-ATP enzyme and Mg++-ATP enzyme in Brain tissue, observe the changes of hippocampus in brain tissue, the comprehensive weight method was used to evaluate the efficacy of each component finally. Results: The contents of total flavonoids and total saponins in the active part of Mao Dongqing can significantly improve the pathological changes of brain tissue in rats, improve the activity of Na+-K+-ATP enzyme, Ca++-ATP enzyme and Mg++-ATP enzyme in the brain of rats, and reduce the level of glutamic acid in serum. The most significant of the contents was the ratio of 10:6. Conclusion: The different proportions of total flavonoids and total saponins in the active part of Mao Dongqing all has a better effect on the rats with TIA, and the ratio of 10:6 is the best active component for preventing and controlling TIA. Keywords: Mao Dongqing'stotal flavonoids, Total saponins, TIA animal model, Optimal active ratio components