Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
Shih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG...
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doaj-957e22a807544480b2882f0db0d869012020-11-24T21:19:52ZengDove Medical PressInternational Journal of Nanomedicine1178-20132015-05-012015default3663368521825Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapyTseng SHChou MYChu IMShih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG-dexSPIONs, by conjugation of the anti-epidermal growth factor receptor (EGFR) monoclonal antibody, cetuximab, to dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) via periodate oxidation. Approximately 31 antibody molecules were conjugated to each nanoparticle. Cet-PEG-dexSPIONs specifically bind to EGFR-expressing tumor cells and enhance image contrast on magnetic resonance imaging. Cet-PEG-dexSPION-treated A431 cells showed significant inhibition of epidermal growth factor-induced EGFR phosphorylation and enhancement of EGFR internalization and degradation. In addition, a significant increase in apoptosis was detected in EGFR-overexpressing cell lines, A431 and 32D/EGFR, after 24 hours of incubation at 37°C with cet-PEG-dexSPIONs compared with cetuximab alone. The antibody-dependent cell-mediated cytotoxicity of cetuximab was observed in cet-PEG-dexSPIONs. The results demonstrated that cet-PEG-dexSPIONs retained the therapeutic effect of cetuximab in addition to having the ability to target and image EGFR-expressing tumors. Cet-PEG-dexSPIONs represent a promising targeted magnetic probe for early detection and treatment of EGFR-expressing tumor cells. Keywords: epidermal growth factor receptor, cetuximab, superparamagnetic iron oxide nanoparticle, magnetic resonance imaging, sodium periodate, polyethylene glycolhttp://www.dovepress.com/cetuximab-conjugated-iron-oxide-nanoparticles-for-cancer-imaging-and-t-peer-reviewed-article-IJN |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tseng SH Chou MY Chu IM |
spellingShingle |
Tseng SH Chou MY Chu IM Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy International Journal of Nanomedicine |
author_facet |
Tseng SH Chou MY Chu IM |
author_sort |
Tseng SH |
title |
Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
title_short |
Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
title_full |
Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
title_fullStr |
Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
title_full_unstemmed |
Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
title_sort |
cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1178-2013 |
publishDate |
2015-05-01 |
description |
Shih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG-dexSPIONs, by conjugation of the anti-epidermal growth factor receptor (EGFR) monoclonal antibody, cetuximab, to dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) via periodate oxidation. Approximately 31 antibody molecules were conjugated to each nanoparticle. Cet-PEG-dexSPIONs specifically bind to EGFR-expressing tumor cells and enhance image contrast on magnetic resonance imaging. Cet-PEG-dexSPION-treated A431 cells showed significant inhibition of epidermal growth factor-induced EGFR phosphorylation and enhancement of EGFR internalization and degradation. In addition, a significant increase in apoptosis was detected in EGFR-overexpressing cell lines, A431 and 32D/EGFR, after 24 hours of incubation at 37°C with cet-PEG-dexSPIONs compared with cetuximab alone. The antibody-dependent cell-mediated cytotoxicity of cetuximab was observed in cet-PEG-dexSPIONs. The results demonstrated that cet-PEG-dexSPIONs retained the therapeutic effect of cetuximab in addition to having the ability to target and image EGFR-expressing tumors. Cet-PEG-dexSPIONs represent a promising targeted magnetic probe for early detection and treatment of EGFR-expressing tumor cells. Keywords: epidermal growth factor receptor, cetuximab, superparamagnetic iron oxide nanoparticle, magnetic resonance imaging, sodium periodate, polyethylene glycol |
url |
http://www.dovepress.com/cetuximab-conjugated-iron-oxide-nanoparticles-for-cancer-imaging-and-t-peer-reviewed-article-IJN |
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