Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation.
BACKGROUND:Electrical vagal nerve stimulation (VNS) has been used for years to treat patients with drug-resistant epilepsy. This technique also remains under investigation as a specific treatment of patients with Alzheimer's disease. Recently we discovered that VNS induced hippocampal formation...
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doaj-957f211466cf4eb78c4dc22f88383e8e2020-11-25T02:33:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011311e020653210.1371/journal.pone.0206532Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation.Adam BroncelRenata BocianPaulina Kłos-WojtczakJan KonopackiBACKGROUND:Electrical vagal nerve stimulation (VNS) has been used for years to treat patients with drug-resistant epilepsy. This technique also remains under investigation as a specific treatment of patients with Alzheimer's disease. Recently we discovered that VNS induced hippocampal formation (HPC) type II theta rhythm, which is involved in memory consolidation. In the present study, we have extended our previous observation and addressed the neuronal substrate and pharmacological profile of HPC type II theta rhythm induced by VNS in anesthetized rats. METHODS:Male Wistar rats were implanted with a VNS cuff electrode around the left vagus nerve, a tungsten microelectrode for recording the HPC field activity, and a medial septal (MS) cannula for the injection of a local anesthetic, procaine, and muscarinic agents. A direct, brief effect of VNS on the HPC field potential was evaluated before and after medial-septal drug injection. RESULTS:Medial septal injection of local anesthetic, procaine, reversibly abolished VNS-induced HPC theta rhythm. With the use of cholinergic muscarinic agonist and antagonists, we demonstrated that medial septal M1 receptors are involved in the mediation of the VNS effect on HPC theta field potential. CONCLUSION:The MS cholinergic M1 receptor mechanism integrates not only central inputs from the brainstem synchronizing pathway, which underlies the production of HPC type II theta rhythm, but also the input from the vagal afferents in the brain stem.http://europepmc.org/articles/PMC6218045?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adam Broncel Renata Bocian Paulina Kłos-Wojtczak Jan Konopacki |
spellingShingle |
Adam Broncel Renata Bocian Paulina Kłos-Wojtczak Jan Konopacki Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. PLoS ONE |
author_facet |
Adam Broncel Renata Bocian Paulina Kłos-Wojtczak Jan Konopacki |
author_sort |
Adam Broncel |
title |
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
title_short |
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
title_full |
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
title_fullStr |
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
title_full_unstemmed |
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
title_sort |
medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
BACKGROUND:Electrical vagal nerve stimulation (VNS) has been used for years to treat patients with drug-resistant epilepsy. This technique also remains under investigation as a specific treatment of patients with Alzheimer's disease. Recently we discovered that VNS induced hippocampal formation (HPC) type II theta rhythm, which is involved in memory consolidation. In the present study, we have extended our previous observation and addressed the neuronal substrate and pharmacological profile of HPC type II theta rhythm induced by VNS in anesthetized rats. METHODS:Male Wistar rats were implanted with a VNS cuff electrode around the left vagus nerve, a tungsten microelectrode for recording the HPC field activity, and a medial septal (MS) cannula for the injection of a local anesthetic, procaine, and muscarinic agents. A direct, brief effect of VNS on the HPC field potential was evaluated before and after medial-septal drug injection. RESULTS:Medial septal injection of local anesthetic, procaine, reversibly abolished VNS-induced HPC theta rhythm. With the use of cholinergic muscarinic agonist and antagonists, we demonstrated that medial septal M1 receptors are involved in the mediation of the VNS effect on HPC theta field potential. CONCLUSION:The MS cholinergic M1 receptor mechanism integrates not only central inputs from the brainstem synchronizing pathway, which underlies the production of HPC type II theta rhythm, but also the input from the vagal afferents in the brain stem. |
url |
http://europepmc.org/articles/PMC6218045?pdf=render |
work_keys_str_mv |
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