Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease
An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Genética
2011-01-01
|
Series: | Genetics and Molecular Biology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100001 |
id |
doaj-9599d825d6404bf9a780d67d165e46ed |
---|---|
record_format |
Article |
spelling |
doaj-9599d825d6404bf9a780d67d165e46ed2020-11-25T02:13:00ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-0134115Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's diseaseAlex Augusto VendraminiRoger Willian de LábioLucas Trevizani RasmussenNathali Mattiuzo dos ReisThais MinettPaulo Henrique Ferreira BertolucciMarcela Augusta de Souza PinhelDorotéia Rossi Silva SouzaDiego Robles MazzottiMarília de Arruda Cardoso SmithSpencer Luiz Marques PayãoAn inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8 -251T > A and IL-1α-889C > T were not found to be risk factors for AD.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100001IL-8IL-1αAlzheimer's DiseaseAPOEinflammatory response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alex Augusto Vendramini Roger Willian de Lábio Lucas Trevizani Rasmussen Nathali Mattiuzo dos Reis Thais Minett Paulo Henrique Ferreira Bertolucci Marcela Augusta de Souza Pinhel Dorotéia Rossi Silva Souza Diego Robles Mazzotti Marília de Arruda Cardoso Smith Spencer Luiz Marques Payão |
spellingShingle |
Alex Augusto Vendramini Roger Willian de Lábio Lucas Trevizani Rasmussen Nathali Mattiuzo dos Reis Thais Minett Paulo Henrique Ferreira Bertolucci Marcela Augusta de Souza Pinhel Dorotéia Rossi Silva Souza Diego Robles Mazzotti Marília de Arruda Cardoso Smith Spencer Luiz Marques Payão Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease Genetics and Molecular Biology IL-8 IL-1α Alzheimer's Disease APOE inflammatory response |
author_facet |
Alex Augusto Vendramini Roger Willian de Lábio Lucas Trevizani Rasmussen Nathali Mattiuzo dos Reis Thais Minett Paulo Henrique Ferreira Bertolucci Marcela Augusta de Souza Pinhel Dorotéia Rossi Silva Souza Diego Robles Mazzotti Marília de Arruda Cardoso Smith Spencer Luiz Marques Payão |
author_sort |
Alex Augusto Vendramini |
title |
Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease |
title_short |
Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease |
title_full |
Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease |
title_fullStr |
Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease |
title_full_unstemmed |
Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease |
title_sort |
interleukin-8-251t > a, interleukin-1α-889c > t and apolipoprotein e polymorphisms in alzheimer's disease |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2011-01-01 |
description |
An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8 -251T > A and IL-1α-889C > T were not found to be risk factors for AD. |
topic |
IL-8 IL-1α Alzheimer's Disease APOE inflammatory response |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100001 |
work_keys_str_mv |
AT alexaugustovendramini interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT rogerwilliandelabio interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT lucastrevizanirasmussen interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT nathalimattiuzodosreis interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT thaisminett interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT paulohenriqueferreirabertolucci interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT marcelaaugustadesouzapinhel interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT doroteiarossisilvasouza interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT diegoroblesmazzotti interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT mariliadearrudacardososmith interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease AT spencerluizmarquespayao interleukin8251tainterleukin1945889ctandapolipoproteinepolymorphismsinalzheimersdisease |
_version_ |
1724906904345378816 |