Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite
Abstract Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino a...
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doaj-95a90c11be5c41fb95193a82ed8f67d62021-04-04T11:27:50ZengBMCClinical and Molecular Allergy1476-79612021-04-011911910.1186/s12948-021-00142-zTropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust miteSu Duan0Limin Zhao1Yuling Zhang2Nan Zhang3Ming Zheng4Qiqi Wang5Xu Zhang6Xiangdong Wang7Sun Ying8Claus Bachert9Luo Zhang10Feng Lan11Beijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyUpper Airways Research Laboratory, ENT Department, Ghent UniversityBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyDepartment of Immunology, School of Basic Medical Science, Capital medical UniversityUpper Airways Research Laboratory, ENT Department, Ghent UniversityBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyAbstract Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively. Results Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5. Conclusions Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation.https://doi.org/10.1186/s12948-021-00142-zCross-reactivityHouse dust miteMugwort |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Su Duan Limin Zhao Yuling Zhang Nan Zhang Ming Zheng Qiqi Wang Xu Zhang Xiangdong Wang Sun Ying Claus Bachert Luo Zhang Feng Lan |
spellingShingle |
Su Duan Limin Zhao Yuling Zhang Nan Zhang Ming Zheng Qiqi Wang Xu Zhang Xiangdong Wang Sun Ying Claus Bachert Luo Zhang Feng Lan Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite Clinical and Molecular Allergy Cross-reactivity House dust mite Mugwort |
author_facet |
Su Duan Limin Zhao Yuling Zhang Nan Zhang Ming Zheng Qiqi Wang Xu Zhang Xiangdong Wang Sun Ying Claus Bachert Luo Zhang Feng Lan |
author_sort |
Su Duan |
title |
Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite |
title_short |
Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite |
title_full |
Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite |
title_fullStr |
Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite |
title_full_unstemmed |
Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite |
title_sort |
tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-th2 response in allergic rhinitis patients sensitized to house dust mite |
publisher |
BMC |
series |
Clinical and Molecular Allergy |
issn |
1476-7961 |
publishDate |
2021-04-01 |
description |
Abstract Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively. Results Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5. Conclusions Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation. |
topic |
Cross-reactivity House dust mite Mugwort |
url |
https://doi.org/10.1186/s12948-021-00142-z |
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