Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum

Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will hel...

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Main Authors: Yang Yu, Jifen Xiao, Jiao Du, Yuheng Yang, Chaowei Bi, Ling Qing
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-11-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01787/full
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spelling doaj-95df63bd89064c23b8e39e44abf001432020-11-24T22:43:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01787229027Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorumYang Yu0Jifen Xiao1Jiao Du2Yuheng Yang3Chaowei Bi4Ling Qing5College of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversitySclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-β-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of S. sclerotiorum.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01787/fullVirulenceGene DeletionCarpogenic germinationSclerotinia sclerotiorumSclerotiaendo-β-1 4-xylanase
collection DOAJ
language English
format Article
sources DOAJ
author Yang Yu
Jifen Xiao
Jiao Du
Yuheng Yang
Chaowei Bi
Ling Qing
spellingShingle Yang Yu
Jifen Xiao
Jiao Du
Yuheng Yang
Chaowei Bi
Ling Qing
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
Frontiers in Microbiology
Virulence
Gene Deletion
Carpogenic germination
Sclerotinia sclerotiorum
Sclerotia
endo-β-1 4-xylanase
author_facet Yang Yu
Jifen Xiao
Jiao Du
Yuheng Yang
Chaowei Bi
Ling Qing
author_sort Yang Yu
title Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
title_short Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
title_full Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
title_fullStr Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
title_full_unstemmed Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
title_sort disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of sclerotinia sclerotiorum
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2016-11-01
description Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-β-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of S. sclerotiorum.
topic Virulence
Gene Deletion
Carpogenic germination
Sclerotinia sclerotiorum
Sclerotia
endo-β-1 4-xylanase
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01787/full
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AT jifenxiao disruptionofthegeneencodingendob14xylanaseaffectsthegrowthandvirulenceofsclerotiniasclerotiorum
AT jiaodu disruptionofthegeneencodingendob14xylanaseaffectsthegrowthandvirulenceofsclerotiniasclerotiorum
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