Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum
Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will hel...
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doaj-95df63bd89064c23b8e39e44abf001432020-11-24T22:43:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01787229027Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorumYang Yu0Jifen Xiao1Jiao Du2Yuheng Yang3Chaowei Bi4Ling Qing5College of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversityCollege of Plant Protection, Southwest UniversitySclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-β-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of S. sclerotiorum.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01787/fullVirulenceGene DeletionCarpogenic germinationSclerotinia sclerotiorumSclerotiaendo-β-1 4-xylanase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Yu Jifen Xiao Jiao Du Yuheng Yang Chaowei Bi Ling Qing |
spellingShingle |
Yang Yu Jifen Xiao Jiao Du Yuheng Yang Chaowei Bi Ling Qing Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum Frontiers in Microbiology Virulence Gene Deletion Carpogenic germination Sclerotinia sclerotiorum Sclerotia endo-β-1 4-xylanase |
author_facet |
Yang Yu Jifen Xiao Jiao Du Yuheng Yang Chaowei Bi Ling Qing |
author_sort |
Yang Yu |
title |
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum |
title_short |
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum |
title_full |
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum |
title_fullStr |
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum |
title_full_unstemmed |
Disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of Sclerotinia sclerotiorum |
title_sort |
disruption of the gene encoding endo-β-1, 4-xylanase affects the growth and virulence of sclerotinia sclerotiorum |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-11-01 |
description |
Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWEDs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-β-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of S. sclerotiorum. |
topic |
Virulence Gene Deletion Carpogenic germination Sclerotinia sclerotiorum Sclerotia endo-β-1 4-xylanase |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01787/full |
work_keys_str_mv |
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