Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).

OBJECTIVE:The objective of this study is to determine the prevalence of rectal carriage of plasmid- and chromosome-encoded AmpC β-lactamase-producing Escherichia coli and Klebsiella spp. in patients in a Dutch teaching hospital between 2013 and 2016. METHODS:Between 2013 and 2016, hospital-wide year...

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Main Authors: Evert den Drijver, Jaco J Verweij, Carlo Verhulst, Stijn Oome, Joke Soer, Ina Willemsen, Eefje J A Schrauwen, Marjolein F Q Kluytmans-van den Bergh, Jan A J W Kluytmans
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6166941?pdf=render
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spelling doaj-95e683fa5f2742e2a3c04d990cefa7a62020-11-25T02:35:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020486410.1371/journal.pone.0204864Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).Evert den DrijverJaco J VerweijCarlo VerhulstStijn OomeJoke SoerIna WillemsenEefje J A SchrauwenMarjolein F Q Kluytmans-van den BerghJan A J W KluytmansOBJECTIVE:The objective of this study is to determine the prevalence of rectal carriage of plasmid- and chromosome-encoded AmpC β-lactamase-producing Escherichia coli and Klebsiella spp. in patients in a Dutch teaching hospital between 2013 and 2016. METHODS:Between 2013 and 2016, hospital-wide yearly prevalence surveys were performed to determine the prevalence of AmpC β-lactamase-producing E. coli and Klebsiella spp. rectal carriage. Rectal swabs were taken and cultured using an enrichment broth and selective agar plates. All E. coli and Klebsiella spp. isolates were screened for production of AmpC β-lactamase using phenotypic confirmation tests and for the presence of plasmid-encoded AmpC (pAmpC) genes. E. coli isolates were screened for chromosome-encoded AmpC (cAmpC) promoter/attenuator alterations. RESULTS:Fifty (2.4%) of 2,126 evaluable patients were identified as rectal carrier of AmpC β-lactamase-producing E. coli. No carriage of AmpC β-lactamase producing Klebsiella spp. was found. Nineteen (0.9%) patients harboured isolates with pAmpC genes and 30 (1,4%) patients harboured isolates with cAmpC promoter/attenuator alterations associated with AmpC β-lactamase overproduction. For one isolate, no pAmpC genes or cAmpC promotor/attenuator alterations could be identified. During the study period, a statistically significant decline in the prevalence of rectal carriage with E. coli with cAmpC promotor/attenuator alterations was found (p = 0.012). The prevalence of pAmpC remained stable over the years. CONCLUSIONS:The prevalence of rectal carriage of AmpC-producing E. coli and Klebsiella spp. in patients in Dutch hospitals is low and a declining trend was observed for E. coli with cAmpC promotor/attenuator alterations.http://europepmc.org/articles/PMC6166941?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Evert den Drijver
Jaco J Verweij
Carlo Verhulst
Stijn Oome
Joke Soer
Ina Willemsen
Eefje J A Schrauwen
Marjolein F Q Kluytmans-van den Bergh
Jan A J W Kluytmans
spellingShingle Evert den Drijver
Jaco J Verweij
Carlo Verhulst
Stijn Oome
Joke Soer
Ina Willemsen
Eefje J A Schrauwen
Marjolein F Q Kluytmans-van den Bergh
Jan A J W Kluytmans
Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
PLoS ONE
author_facet Evert den Drijver
Jaco J Verweij
Carlo Verhulst
Stijn Oome
Joke Soer
Ina Willemsen
Eefje J A Schrauwen
Marjolein F Q Kluytmans-van den Bergh
Jan A J W Kluytmans
author_sort Evert den Drijver
title Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
title_short Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
title_full Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
title_fullStr Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
title_full_unstemmed Decline in AmpC β-lactamase-producing Escherichia coli in a Dutch teaching hospital (2013-2016).
title_sort decline in ampc β-lactamase-producing escherichia coli in a dutch teaching hospital (2013-2016).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2018-01-01
description OBJECTIVE:The objective of this study is to determine the prevalence of rectal carriage of plasmid- and chromosome-encoded AmpC β-lactamase-producing Escherichia coli and Klebsiella spp. in patients in a Dutch teaching hospital between 2013 and 2016. METHODS:Between 2013 and 2016, hospital-wide yearly prevalence surveys were performed to determine the prevalence of AmpC β-lactamase-producing E. coli and Klebsiella spp. rectal carriage. Rectal swabs were taken and cultured using an enrichment broth and selective agar plates. All E. coli and Klebsiella spp. isolates were screened for production of AmpC β-lactamase using phenotypic confirmation tests and for the presence of plasmid-encoded AmpC (pAmpC) genes. E. coli isolates were screened for chromosome-encoded AmpC (cAmpC) promoter/attenuator alterations. RESULTS:Fifty (2.4%) of 2,126 evaluable patients were identified as rectal carrier of AmpC β-lactamase-producing E. coli. No carriage of AmpC β-lactamase producing Klebsiella spp. was found. Nineteen (0.9%) patients harboured isolates with pAmpC genes and 30 (1,4%) patients harboured isolates with cAmpC promoter/attenuator alterations associated with AmpC β-lactamase overproduction. For one isolate, no pAmpC genes or cAmpC promotor/attenuator alterations could be identified. During the study period, a statistically significant decline in the prevalence of rectal carriage with E. coli with cAmpC promotor/attenuator alterations was found (p = 0.012). The prevalence of pAmpC remained stable over the years. CONCLUSIONS:The prevalence of rectal carriage of AmpC-producing E. coli and Klebsiella spp. in patients in Dutch hospitals is low and a declining trend was observed for E. coli with cAmpC promotor/attenuator alterations.
url http://europepmc.org/articles/PMC6166941?pdf=render
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