Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing
Secondary bone fracture healing is a physiological process that leads to functional tissue regeneration via endochondral bone formation. In vivo studies have demonstrated that early mobilization and the application of mechanical loads enhances the process of fracture healing. However, the influence...
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doaj-95f5358646fa49228966b65bae189e2b2020-11-24T21:29:17ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852015-02-01310.3389/fbioe.2015.00010127056Novel perfused compression bioreactor system as an in vitro model to investigate fracture healingWaldemar eHoffmann0Waldemar eHoffmann1Sandra eFeliciano2Ivan eMartin3Michael ede Wild4David eWendt5University Hospital BaselUniversity of Applied Sciences Northwestern SwitzerlandUniversity Hospital BaselUniversity Hospital BaselUniversity of Applied Sciences Northwestern SwitzerlandUniversity Hospital BaselSecondary bone fracture healing is a physiological process that leads to functional tissue regeneration via endochondral bone formation. In vivo studies have demonstrated that early mobilization and the application of mechanical loads enhances the process of fracture healing. However, the influence of specific mechanical stimuli and particular effects during specific phases of fracture healing remain to be elucidated. In this work, we have developed and provided proof-of-concept of an in vitro human organotypic model of physiological loading of a cartilage callus, based on a novel perfused compression bioreactor system (PCB). We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells towards late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments.http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00010/fullFracture HealingHypertrophybioreactorin vitro modelmechanical loading |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Waldemar eHoffmann Waldemar eHoffmann Sandra eFeliciano Ivan eMartin Michael ede Wild David eWendt |
spellingShingle |
Waldemar eHoffmann Waldemar eHoffmann Sandra eFeliciano Ivan eMartin Michael ede Wild David eWendt Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing Frontiers in Bioengineering and Biotechnology Fracture Healing Hypertrophy bioreactor in vitro model mechanical loading |
author_facet |
Waldemar eHoffmann Waldemar eHoffmann Sandra eFeliciano Ivan eMartin Michael ede Wild David eWendt |
author_sort |
Waldemar eHoffmann |
title |
Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
title_short |
Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
title_full |
Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
title_fullStr |
Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
title_full_unstemmed |
Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
title_sort |
novel perfused compression bioreactor system as an in vitro model to investigate fracture healing |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2015-02-01 |
description |
Secondary bone fracture healing is a physiological process that leads to functional tissue regeneration via endochondral bone formation. In vivo studies have demonstrated that early mobilization and the application of mechanical loads enhances the process of fracture healing. However, the influence of specific mechanical stimuli and particular effects during specific phases of fracture healing remain to be elucidated. In this work, we have developed and provided proof-of-concept of an in vitro human organotypic model of physiological loading of a cartilage callus, based on a novel perfused compression bioreactor system (PCB). We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells towards late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments. |
topic |
Fracture Healing Hypertrophy bioreactor in vitro model mechanical loading |
url |
http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00010/full |
work_keys_str_mv |
AT waldemarehoffmann novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing AT waldemarehoffmann novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing AT sandraefeliciano novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing AT ivanemartin novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing AT michaeledewild novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing AT davidewendt novelperfusedcompressionbioreactorsystemasaninvitromodeltoinvestigatefracturehealing |
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