Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management
Magnetic resonance imaging (MRI) is the gold standard for glioblastoma (GBM) patient evaluation. Additional non-invasive diagnostic modalities are needed. GBM is heavily infiltrated with tumor-associated macrophages (TAMs) that can be found in peripheral blood. FKBP51s supports alternative-macrophag...
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doaj-961ae49f77a9487e97a71555bcb5e6602021-04-06T23:05:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01223797379710.3390/ijms22073797Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM ManagementCarolina Giordano0Giovanni Sabatino1Simona Romano2Giuseppe Maria Della Pepa3Martina Tufano4Quintino Giorgio D’Alessandris5Simone Cottonaro6Marco Gessi7Mario Balducci8Maria Fiammetta Romano9Alessandro Olivi10Simona Gaudino11Cesare Colosimo12Università Cattolica S.Cuore, 00168 Roma, ItalyUOC Neurochirurgia, Istituto di Neurochirurgia, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S.Cuore, 00168 Roma, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, via Pansini, 5, 80131 Napoli, ItalyUOC Neurochirurgia, Istituto di Neurochirurgia, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S.Cuore, 00168 Roma, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, via Pansini, 5, 80131 Napoli, ItalyUOC Neurochirurgia, Istituto di Neurochirurgia, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S.Cuore, 00168 Roma, ItalyUniversità Cattolica S.Cuore, 00168 Roma, ItalyUOS di Neuropatologia, UOC Anatomia Patologica, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S. Cuore, 00168 Roma, ItalyUOC di Radioterapia Oncologica, Dipartimento di Diagnostica per Immagini, Radioterapia Oncologica ed Ematologia, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S.Cuore, 00168 Roma, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, via Pansini, 5, 80131 Napoli, ItalyUOC Neurochirurgia, Istituto di Neurochirurgia, Fondazione Policlinico Universitario “A.Gemelli” IRCCS, Università Cattolica S.Cuore, 00168 Roma, ItalyUniversità Cattolica S.Cuore, 00168 Roma, ItalyUniversità Cattolica S.Cuore, 00168 Roma, ItalyMagnetic resonance imaging (MRI) is the gold standard for glioblastoma (GBM) patient evaluation. Additional non-invasive diagnostic modalities are needed. GBM is heavily infiltrated with tumor-associated macrophages (TAMs) that can be found in peripheral blood. FKBP51s supports alternative-macrophage polarization. Herein, we assessed FKBP51s expression in circulating monocytes from 14 GBM patients. The M2 monocyte phenotype was investigated by qPCR and flow cytometry using antibodies against PD-L1, CD163, FKBP51s, and CD14. MRI assessed morphologic features of the tumors that were aligned to flow cytometry data. PD-L1 expression on circulating monocytes correlated with MRI tumor necrosis score. A wider expansion in circulating CD163/monocytes was measured. These monocytes resulted in a dramatic decrease in patients with an MRI diagnosis of complete but not partial surgical removal of the tumor. Importantly, in patients with residual tumor, most of the peripheral monocytes that in the preoperative stage were CD163/FKBP51s− had turned into CD163/FKBP51s+. After Stupp therapy, CD163/FKBP51s+ monocytes were almost absent in a case of pseudoprogression, while two patients with stable or true disease progression showed sustained levels in such circulating monocytes. Our work provides preliminary but meaningful and novel results that deserve to be confirmed in a larger patient cohort, in support of potential usefulness in GBM monitoring of CD163/FKBP51s/CD14 immunophenotype in adjunct to MRI.https://www.mdpi.com/1422-0067/22/7/3797glioblastoma multiformeMRIpseudoprogressionliquid biopsyFKBP51s |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carolina Giordano Giovanni Sabatino Simona Romano Giuseppe Maria Della Pepa Martina Tufano Quintino Giorgio D’Alessandris Simone Cottonaro Marco Gessi Mario Balducci Maria Fiammetta Romano Alessandro Olivi Simona Gaudino Cesare Colosimo |
spellingShingle |
Carolina Giordano Giovanni Sabatino Simona Romano Giuseppe Maria Della Pepa Martina Tufano Quintino Giorgio D’Alessandris Simone Cottonaro Marco Gessi Mario Balducci Maria Fiammetta Romano Alessandro Olivi Simona Gaudino Cesare Colosimo Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management International Journal of Molecular Sciences glioblastoma multiforme MRI pseudoprogression liquid biopsy FKBP51s |
author_facet |
Carolina Giordano Giovanni Sabatino Simona Romano Giuseppe Maria Della Pepa Martina Tufano Quintino Giorgio D’Alessandris Simone Cottonaro Marco Gessi Mario Balducci Maria Fiammetta Romano Alessandro Olivi Simona Gaudino Cesare Colosimo |
author_sort |
Carolina Giordano |
title |
Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management |
title_short |
Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management |
title_full |
Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management |
title_fullStr |
Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management |
title_full_unstemmed |
Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management |
title_sort |
combining magnetic resonance imaging with systemic monocyte evaluation for the implementation of gbm management |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-04-01 |
description |
Magnetic resonance imaging (MRI) is the gold standard for glioblastoma (GBM) patient evaluation. Additional non-invasive diagnostic modalities are needed. GBM is heavily infiltrated with tumor-associated macrophages (TAMs) that can be found in peripheral blood. FKBP51s supports alternative-macrophage polarization. Herein, we assessed FKBP51s expression in circulating monocytes from 14 GBM patients. The M2 monocyte phenotype was investigated by qPCR and flow cytometry using antibodies against PD-L1, CD163, FKBP51s, and CD14. MRI assessed morphologic features of the tumors that were aligned to flow cytometry data. PD-L1 expression on circulating monocytes correlated with MRI tumor necrosis score. A wider expansion in circulating CD163/monocytes was measured. These monocytes resulted in a dramatic decrease in patients with an MRI diagnosis of complete but not partial surgical removal of the tumor. Importantly, in patients with residual tumor, most of the peripheral monocytes that in the preoperative stage were CD163/FKBP51s− had turned into CD163/FKBP51s+. After Stupp therapy, CD163/FKBP51s+ monocytes were almost absent in a case of pseudoprogression, while two patients with stable or true disease progression showed sustained levels in such circulating monocytes. Our work provides preliminary but meaningful and novel results that deserve to be confirmed in a larger patient cohort, in support of potential usefulness in GBM monitoring of CD163/FKBP51s/CD14 immunophenotype in adjunct to MRI. |
topic |
glioblastoma multiforme MRI pseudoprogression liquid biopsy FKBP51s |
url |
https://www.mdpi.com/1422-0067/22/7/3797 |
work_keys_str_mv |
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