Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in tran...
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doaj-96201176a2504ccfab33d6de57d480302020-11-24T20:48:09ZengMDPI AGCells2073-44092019-08-018882210.3390/cells8080822cells8080822Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late BlightYu-Hui Hong0Jun Meng1Xiao-Li He2Yuan-Yuan Zhang3Yu-Shi Luan4School of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Computer Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaTomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels.https://www.mdpi.com/2073-4409/8/8/822tomatolate blightmiRNAoverexpressionresistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Hui Hong Jun Meng Xiao-Li He Yuan-Yuan Zhang Yu-Shi Luan |
spellingShingle |
Yu-Hui Hong Jun Meng Xiao-Li He Yuan-Yuan Zhang Yu-Shi Luan Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight Cells tomato late blight miRNA overexpression resistance |
author_facet |
Yu-Hui Hong Jun Meng Xiao-Li He Yuan-Yuan Zhang Yu-Shi Luan |
author_sort |
Yu-Hui Hong |
title |
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_short |
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_full |
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_fullStr |
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_full_unstemmed |
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_sort |
overexpression of mir482c in tomato induces enhanced susceptibility to late blight |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-08-01 |
description |
Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels. |
topic |
tomato late blight miRNA overexpression resistance |
url |
https://www.mdpi.com/2073-4409/8/8/822 |
work_keys_str_mv |
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