HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells
Vascular inflammation has been implicated in the progression of cardiovascular diseases such as atherosclerosis. In the present study, we found that HMC05, an extract from eight different herbal mixtures, dose-dependently inhibited tumor necrosis factor-α (TNF-α)-induced adhesion of monocytes to end...
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Online Access: | http://dx.doi.org/10.1093/ecam/nep126 |
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doaj-962f0ea197b043e5ae37e9d6c8f470f02020-11-24T22:43:11ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882011-01-01201110.1093/ecam/nep126974728HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial CellsJong Suk Lee0Su-Young Park1Dinesh Thapa2Ah Ra Kim3Heung-Mook Shin4Jung-Ae Kim5College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of KoreaCollege of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of KoreaCollege of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of KoreaCollege of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of KoreaDepartment of Physiology, College of Oriental Medicine, Dongguk University, Gyoungju 780-714, Republic of KoreaCollege of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of KoreaVascular inflammation has been implicated in the progression of cardiovascular diseases such as atherosclerosis. In the present study, we found that HMC05, an extract from eight different herbal mixtures, dose-dependently inhibited tumor necrosis factor-α (TNF-α)-induced adhesion of monocytes to endothelial cells. Such inhibitory effect of HMC05 correlated with suppressed expression of monocyte chemoattractant protein-1, CC chemokine receptor 2, vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1. In addition, HMC05 significantly inhibited production of reactive oxygen species (ROS) and nuclear factor (NF)-κB activation by TNF-α. Those inhibitory effects of HMC05 (1–10 μg mL−1) on the TNF-α-induced inflammatory event was similar to those of berberine (1–10 μM), which is a major component of HMC05 and one of herbal compounds known to have vasorelaxing and lipid-lowering activities. However, berberine significantly reduced the viability of HUVECs in a time- and concentration-dependent manner. In contrast, HMC05 (1–10 μg ml−1) did not affect the cell viability for up to 48 h treatment. In conclusion, we propose that HMC05 may be a safe and potent herbal formula against vascular inflammation, and its action may be attributable to the inhibition of ROS- and NF-κB-dependent expression of adhesion molecules and chemokines.http://dx.doi.org/10.1093/ecam/nep126 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jong Suk Lee Su-Young Park Dinesh Thapa Ah Ra Kim Heung-Mook Shin Jung-Ae Kim |
spellingShingle |
Jong Suk Lee Su-Young Park Dinesh Thapa Ah Ra Kim Heung-Mook Shin Jung-Ae Kim HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells Evidence-Based Complementary and Alternative Medicine |
author_facet |
Jong Suk Lee Su-Young Park Dinesh Thapa Ah Ra Kim Heung-Mook Shin Jung-Ae Kim |
author_sort |
Jong Suk Lee |
title |
HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells |
title_short |
HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells |
title_full |
HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells |
title_fullStr |
HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells |
title_full_unstemmed |
HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells |
title_sort |
hmc05, herbal formula, inhibits tnf-α-induced inflammatory response in human umbilical vein endothelial cells |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2011-01-01 |
description |
Vascular inflammation has been implicated in the progression of cardiovascular diseases such as atherosclerosis. In the present study, we found that HMC05, an extract from eight different herbal mixtures, dose-dependently inhibited tumor necrosis factor-α (TNF-α)-induced adhesion of monocytes to endothelial cells. Such inhibitory effect of HMC05 correlated with suppressed expression of monocyte chemoattractant protein-1, CC chemokine receptor 2, vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1. In addition, HMC05 significantly inhibited production of reactive oxygen species (ROS) and nuclear factor (NF)-κB activation by TNF-α. Those inhibitory effects of HMC05 (1–10 μg mL−1) on the TNF-α-induced inflammatory event was similar to those of berberine (1–10 μM), which is a major component of HMC05 and one of herbal compounds known to have vasorelaxing and lipid-lowering activities. However, berberine significantly reduced the viability of HUVECs in a time- and concentration-dependent manner. In contrast, HMC05 (1–10 μg ml−1) did not affect the cell viability for up to 48 h treatment. In conclusion, we propose that HMC05 may be a safe and potent herbal formula against vascular inflammation, and its action may be attributable to the inhibition of ROS- and NF-κB-dependent expression of adhesion molecules and chemokines. |
url |
http://dx.doi.org/10.1093/ecam/nep126 |
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