HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates
ABSTRACT: Objectives: The prevalence of carbapenem-resistant Enterobacterales (CRE) has increased rapidly worldwide in the last two decades. CRE infection poses a huge challenge for today's clinical therapy. Rapid and accurate detection of clinical CRE isolates can avoid inappropriate antimicr...
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doaj-963ab3374d104b68b7caa23ef354e4e72021-09-15T04:21:44ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-09-0126272278HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolatesMing Wei0Peng Wang1Shuai Wang2Chunxia Yang3Li Gu4Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of ChinaDepartment of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of ChinaDepartment of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of ChinaDepartment of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of ChinaCorresponding author. Mailing address: Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing 100020, People's Republic of China. Tel.: +86 10 8523 1130; fax: +86 10 8523 1130.; Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of ChinaABSTRACT: Objectives: The prevalence of carbapenem-resistant Enterobacterales (CRE) has increased rapidly worldwide in the last two decades. CRE infection poses a huge challenge for today's clinical therapy. Rapid and accurate detection of clinical CRE isolates can avoid inappropriate antimicrobial treatment and reduce mortality. However, existing detection methods are either time consuming, expensive or inaccurate, making them unable to fully meet clinical demands. In this study, the HB&L system was designed to distinguish CRE from carbapenem-susceptible Enterobacterales (CSE), as it can accelerate the growth of bacteria, detect both carbapenemase-producing CRE (CP-CRE) and non-CP-CRE isolates in real time, and provide time–kill curves. Methods: The broth microdilution method and PCR and sequencing were used as the reference methods to identify CRE and carbapenemase-producing Enterobacterales (CPE) isolates, respectively. Three methods for detecting CRE isolates, including the Carba NP test, modified carbapenem inactivation method (mCIM) and HB&L system, were evaluated. Results: The accuracy of the HB&L system was extremely high with 100% sensitivity and 96.0% specificity at only 6 h of culture time for detecting CRE. Time–kill curves may provide information on effective treatment options for clinicians. This system is superior to the mCIM (20–24 h detection time; 90.6% sensitivity and 96.6% specificity) and Carba NP test (2 h detection time; 85.2% sensitivity and 98.4% specificity), which are only designed to detect CP-CRE. Conclusion: The HB&L system is promising for wide application for detection of clinical CRE in hospitals.http://www.sciencedirect.com/science/article/pii/S2213716521001715HB&L systemCarbapenem-resistant EnterobacteralesRapid phenotypic detectionTime–kill curveCarbapenemase gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming Wei Peng Wang Shuai Wang Chunxia Yang Li Gu |
spellingShingle |
Ming Wei Peng Wang Shuai Wang Chunxia Yang Li Gu HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates Journal of Global Antimicrobial Resistance HB&L system Carbapenem-resistant Enterobacterales Rapid phenotypic detection Time–kill curve Carbapenemase gene |
author_facet |
Ming Wei Peng Wang Shuai Wang Chunxia Yang Li Gu |
author_sort |
Ming Wei |
title |
HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates |
title_short |
HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates |
title_full |
HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates |
title_fullStr |
HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates |
title_full_unstemmed |
HB&L system for rapid phenotypic detection of clinical carbapenem-resistant Enterobacterales isolates |
title_sort |
hb&l system for rapid phenotypic detection of clinical carbapenem-resistant enterobacterales isolates |
publisher |
Elsevier |
series |
Journal of Global Antimicrobial Resistance |
issn |
2213-7165 |
publishDate |
2021-09-01 |
description |
ABSTRACT: Objectives: The prevalence of carbapenem-resistant Enterobacterales (CRE) has increased rapidly worldwide in the last two decades. CRE infection poses a huge challenge for today's clinical therapy. Rapid and accurate detection of clinical CRE isolates can avoid inappropriate antimicrobial treatment and reduce mortality. However, existing detection methods are either time consuming, expensive or inaccurate, making them unable to fully meet clinical demands. In this study, the HB&L system was designed to distinguish CRE from carbapenem-susceptible Enterobacterales (CSE), as it can accelerate the growth of bacteria, detect both carbapenemase-producing CRE (CP-CRE) and non-CP-CRE isolates in real time, and provide time–kill curves. Methods: The broth microdilution method and PCR and sequencing were used as the reference methods to identify CRE and carbapenemase-producing Enterobacterales (CPE) isolates, respectively. Three methods for detecting CRE isolates, including the Carba NP test, modified carbapenem inactivation method (mCIM) and HB&L system, were evaluated. Results: The accuracy of the HB&L system was extremely high with 100% sensitivity and 96.0% specificity at only 6 h of culture time for detecting CRE. Time–kill curves may provide information on effective treatment options for clinicians. This system is superior to the mCIM (20–24 h detection time; 90.6% sensitivity and 96.6% specificity) and Carba NP test (2 h detection time; 85.2% sensitivity and 98.4% specificity), which are only designed to detect CP-CRE. Conclusion: The HB&L system is promising for wide application for detection of clinical CRE in hospitals. |
topic |
HB&L system Carbapenem-resistant Enterobacterales Rapid phenotypic detection Time–kill curve Carbapenemase gene |
url |
http://www.sciencedirect.com/science/article/pii/S2213716521001715 |
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