miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia
Despite the differences in the clinical manifestations of major obstetric syndromes, such as preeclampsia (PE) and intrauterine growth restriction (IUGR), their pathogenesis is based on the dysregulation of proliferation, differentiation, and invasion of cytotrophoblast cells that occur in the devel...
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doaj-964da871bfab4c108370afa03deb05952021-04-20T23:02:04ZengMDPI AGDiagnostics2075-44182021-04-011172972910.3390/diagnostics11040729miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and PreeclampsiaAngelika V. Timofeeva0Ivan S. Fedorov1Alexander G. Brzhozovskiy2Anna E. Bugrova3Vitaliy V. Chagovets4Maria V. Volochaeva5Natalia L. Starodubtseva6Vladimir E. Frankevich7Evgeny N. Nikolaev8Roman G. Shmakov9Gennady T. Sukhikh10Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaLaboratory of Mass Spectrometry, CDISE, Skolkovo Institute of Science and Technology, 121205 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaKulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Ac. Oparina 4, 117997 Moscow, RussiaDespite the differences in the clinical manifestations of major obstetric syndromes, such as preeclampsia (PE) and intrauterine growth restriction (IUGR), their pathogenesis is based on the dysregulation of proliferation, differentiation, and invasion of cytotrophoblast cells that occur in the developing placenta, decidual endometrium, and myometrial parts of the spiral arteries. To understand the similarities and differences in the molecular mechanisms of PE and IUGR, samples of the placental bed and placental tissue were analyzed using protein mass spectrometry and the deep sequencing of small RNAs, followed by validation of the data obtained by quantitative RT-PCR in real time. A comparison of the transcriptome and proteomic profiles in the samples made it possible to conclude that the main changes in the molecular profile in IUGR occur in the placental bed, in contrast to PE, in which the majority of molecular changes occurs in the placenta. In placental bed samples, significant changes in the ratio of miRNA and its potential target gene expression levels were revealed, which were unique for IUGR (miR-30c-5p/VIM, miR-28-3p/VIM, miR-1-3p/ANXA2, miR-30c-5p/FBN1; miR-15b-5p/MYL6), unique for PE (miR-185-3p/FLNA), common for IUGR and PE (miR-30c-5p/YWHAZ and miR-654-3p/FGA), but all associated with abnormality in the hemostatic and vascular systems as well as with an inflammatory process at the fetal‒maternal interface.https://www.mdpi.com/2075-4418/11/4/729intrauterine growth restrictionpreeclampsiasmall for gestational ageplacental bedplacentamiRNA deep sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angelika V. Timofeeva Ivan S. Fedorov Alexander G. Brzhozovskiy Anna E. Bugrova Vitaliy V. Chagovets Maria V. Volochaeva Natalia L. Starodubtseva Vladimir E. Frankevich Evgeny N. Nikolaev Roman G. Shmakov Gennady T. Sukhikh |
spellingShingle |
Angelika V. Timofeeva Ivan S. Fedorov Alexander G. Brzhozovskiy Anna E. Bugrova Vitaliy V. Chagovets Maria V. Volochaeva Natalia L. Starodubtseva Vladimir E. Frankevich Evgeny N. Nikolaev Roman G. Shmakov Gennady T. Sukhikh miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia Diagnostics intrauterine growth restriction preeclampsia small for gestational age placental bed placenta miRNA deep sequencing |
author_facet |
Angelika V. Timofeeva Ivan S. Fedorov Alexander G. Brzhozovskiy Anna E. Bugrova Vitaliy V. Chagovets Maria V. Volochaeva Natalia L. Starodubtseva Vladimir E. Frankevich Evgeny N. Nikolaev Roman G. Shmakov Gennady T. Sukhikh |
author_sort |
Angelika V. Timofeeva |
title |
miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia |
title_short |
miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia |
title_full |
miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia |
title_fullStr |
miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia |
title_full_unstemmed |
miRNAs and Their Gene Targets—A Clue to Differentiate Pregnancies with Small for Gestational Age Newborns, Intrauterine Growth Restriction, and Preeclampsia |
title_sort |
mirnas and their gene targets—a clue to differentiate pregnancies with small for gestational age newborns, intrauterine growth restriction, and preeclampsia |
publisher |
MDPI AG |
series |
Diagnostics |
issn |
2075-4418 |
publishDate |
2021-04-01 |
description |
Despite the differences in the clinical manifestations of major obstetric syndromes, such as preeclampsia (PE) and intrauterine growth restriction (IUGR), their pathogenesis is based on the dysregulation of proliferation, differentiation, and invasion of cytotrophoblast cells that occur in the developing placenta, decidual endometrium, and myometrial parts of the spiral arteries. To understand the similarities and differences in the molecular mechanisms of PE and IUGR, samples of the placental bed and placental tissue were analyzed using protein mass spectrometry and the deep sequencing of small RNAs, followed by validation of the data obtained by quantitative RT-PCR in real time. A comparison of the transcriptome and proteomic profiles in the samples made it possible to conclude that the main changes in the molecular profile in IUGR occur in the placental bed, in contrast to PE, in which the majority of molecular changes occurs in the placenta. In placental bed samples, significant changes in the ratio of miRNA and its potential target gene expression levels were revealed, which were unique for IUGR (miR-30c-5p/VIM, miR-28-3p/VIM, miR-1-3p/ANXA2, miR-30c-5p/FBN1; miR-15b-5p/MYL6), unique for PE (miR-185-3p/FLNA), common for IUGR and PE (miR-30c-5p/YWHAZ and miR-654-3p/FGA), but all associated with abnormality in the hemostatic and vascular systems as well as with an inflammatory process at the fetal‒maternal interface. |
topic |
intrauterine growth restriction preeclampsia small for gestational age placental bed placenta miRNA deep sequencing |
url |
https://www.mdpi.com/2075-4418/11/4/729 |
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