Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees
Serine proteases are essential metabolic enzymes in the midgut of many pests, including the red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, which has a significant impact economically, environmentally and socially worldwide especially in the middle east. Some methods have been used to mana...
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doaj-9660351c8b4b4d9380005ef8b752937e2020-11-25T02:54:22ZengElsevierSaudi Journal of Biological Sciences1319-562X2020-10-01271028032808Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm treesRaha Orfali0Arwa Binsuwaileh1Hanan Abu Al-Ala'a2Saja Bane-Gamea3Nada Zaidan4Mahmoud Abdelazim5Mohamad Alhasan Ismael6Shagufta Perveen7Najwa Majrashi8Khulud Alluhayb9Razan S. Orfali10Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia; Corresponding authors.Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaChair of Date Palm Research, Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaChair of Protein Research, College of Science, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaNational Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi ArabiaNational Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi ArabiaChapman University, School of Pharmacy, 9401 Jeronimo Rd, Irvine, CA 92618, USA; Corresponding authors.Serine proteases are essential metabolic enzymes in the midgut of many pests, including the red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, which has a significant impact economically, environmentally and socially worldwide especially in the middle east. Some methods have been used to manage this pest such as trapping of RPW with pheromones, chemicals, and X-rays. However, these methods are costly, not effective and negatively impact the human. The main objective of this study is to contribute to the discovery of an eco-friendly pesticide to eradicate this infection by using serine protease inhibitors (SPIs) extracted from different parts of plant resources. In this research, both in vitro and in vivo effects of SPIs activity against RPW were examined. The protease inhibitors (PIs) activity was recorded in the crude extract that was isolated from the date’s kernel (DKE), host and Calotropis latex (CLE), non-host. These PIs were partially purified by ammonium sulfate precipitation. The midgut tissue of RPW was extracted and analyzed for protases activity assay. PIs assays were consistent with the increased in the inhibitory activity against the midgut proteases after treatment with a DKE and CLE. The reduction of gut proteases by DKE solution and CLE was 39%, 18%, respectively. Partially purified DKE showed the most prominent inhibition pattern of protease activity of the gut extract. While, latex exhibited acute toxicity, imparting the least LC50 (5.132 mg/mL) against RPW larvae. Taken together, these findings provide evidence for the hypothesis that SPIs activity may play an important role in enhancing the mortality of RPW and relieving the toxicity of insecticide in palm trees.http://www.sciencedirect.com/science/article/pii/S1319562X20302886Red palm weevilRhynchophorus ferrugineusProtease inhibitorsDate’s kernelCalotropis latex |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raha Orfali Arwa Binsuwaileh Hanan Abu Al-Ala'a Saja Bane-Gamea Nada Zaidan Mahmoud Abdelazim Mohamad Alhasan Ismael Shagufta Perveen Najwa Majrashi Khulud Alluhayb Razan S. Orfali |
spellingShingle |
Raha Orfali Arwa Binsuwaileh Hanan Abu Al-Ala'a Saja Bane-Gamea Nada Zaidan Mahmoud Abdelazim Mohamad Alhasan Ismael Shagufta Perveen Najwa Majrashi Khulud Alluhayb Razan S. Orfali Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees Saudi Journal of Biological Sciences Red palm weevil Rhynchophorus ferrugineus Protease inhibitors Date’s kernel Calotropis latex |
author_facet |
Raha Orfali Arwa Binsuwaileh Hanan Abu Al-Ala'a Saja Bane-Gamea Nada Zaidan Mahmoud Abdelazim Mohamad Alhasan Ismael Shagufta Perveen Najwa Majrashi Khulud Alluhayb Razan S. Orfali |
author_sort |
Raha Orfali |
title |
Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees |
title_short |
Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees |
title_full |
Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees |
title_fullStr |
Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees |
title_full_unstemmed |
Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees |
title_sort |
production of a biopesticide on host and non-host serine protease inhibitors for red palm weevil in palm trees |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2020-10-01 |
description |
Serine proteases are essential metabolic enzymes in the midgut of many pests, including the red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, which has a significant impact economically, environmentally and socially worldwide especially in the middle east. Some methods have been used to manage this pest such as trapping of RPW with pheromones, chemicals, and X-rays. However, these methods are costly, not effective and negatively impact the human. The main objective of this study is to contribute to the discovery of an eco-friendly pesticide to eradicate this infection by using serine protease inhibitors (SPIs) extracted from different parts of plant resources. In this research, both in vitro and in vivo effects of SPIs activity against RPW were examined. The protease inhibitors (PIs) activity was recorded in the crude extract that was isolated from the date’s kernel (DKE), host and Calotropis latex (CLE), non-host. These PIs were partially purified by ammonium sulfate precipitation. The midgut tissue of RPW was extracted and analyzed for protases activity assay. PIs assays were consistent with the increased in the inhibitory activity against the midgut proteases after treatment with a DKE and CLE. The reduction of gut proteases by DKE solution and CLE was 39%, 18%, respectively. Partially purified DKE showed the most prominent inhibition pattern of protease activity of the gut extract. While, latex exhibited acute toxicity, imparting the least LC50 (5.132 mg/mL) against RPW larvae. Taken together, these findings provide evidence for the hypothesis that SPIs activity may play an important role in enhancing the mortality of RPW and relieving the toxicity of insecticide in palm trees. |
topic |
Red palm weevil Rhynchophorus ferrugineus Protease inhibitors Date’s kernel Calotropis latex |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X20302886 |
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