Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth)
Evaluation of an in vitro mycorrhization system of blackberry plants (Rubus glaucus, Benth). Objective. Obtain an in vitromycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). Materials and methods. We usedspores and root fragments with vesicles of Arbuscu...
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Pontificia Universidad Javeriana
2012-08-01
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doaj-9667f4d8513e4ea39da3773b3dcad2b32020-11-24T22:54:23ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522012-08-01172140151Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth)Víctor Manuel Núñez-ZaranteGabriel Roveda-HoyosMarcela Franco-CorreaMaría Margarita Ramírez-GómezUrley Adrian Pérez-MoncadaEvaluation of an in vitro mycorrhization system of blackberry plants (Rubus glaucus, Benth). Objective. Obtain an in vitromycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). Materials and methods. We usedspores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophicculture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of sporesproduced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measuredthe shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. Results. Theautotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed.Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structurestypical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development ofextraradical mycelium with branched hyphae, and formation of new spores. Conclusion. For the first time, micropropagated blackberryplants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomussp. associated to roots of micropropagated blackberry plantlets.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/3688/2752arbuscular mycorrhizal fungi (AMF)autotrophic cultureRubus glaucus BenthGlomus sp. (GEV02)in vitro mycorrhization. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Víctor Manuel Núñez-Zarante Gabriel Roveda-Hoyos Marcela Franco-Correa María Margarita Ramírez-Gómez Urley Adrian Pérez-Moncada |
spellingShingle |
Víctor Manuel Núñez-Zarante Gabriel Roveda-Hoyos Marcela Franco-Correa María Margarita Ramírez-Gómez Urley Adrian Pérez-Moncada Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) Universitas Scientiarum arbuscular mycorrhizal fungi (AMF) autotrophic culture Rubus glaucus Benth Glomus sp. (GEV02) in vitro mycorrhization. |
author_facet |
Víctor Manuel Núñez-Zarante Gabriel Roveda-Hoyos Marcela Franco-Correa María Margarita Ramírez-Gómez Urley Adrian Pérez-Moncada |
author_sort |
Víctor Manuel Núñez-Zarante |
title |
Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) |
title_short |
Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) |
title_full |
Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) |
title_fullStr |
Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) |
title_full_unstemmed |
Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth) |
title_sort |
evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (rubus glaucus, benth) |
publisher |
Pontificia Universidad Javeriana |
series |
Universitas Scientiarum |
issn |
0122-7483 2027-1352 |
publishDate |
2012-08-01 |
description |
Evaluation of an in vitro mycorrhization system of blackberry plants (Rubus glaucus, Benth). Objective. Obtain an in vitromycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). Materials and methods. We usedspores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophicculture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of sporesproduced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measuredthe shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. Results. Theautotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed.Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structurestypical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development ofextraradical mycelium with branched hyphae, and formation of new spores. Conclusion. For the first time, micropropagated blackberryplants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomussp. associated to roots of micropropagated blackberry plantlets. |
topic |
arbuscular mycorrhizal fungi (AMF) autotrophic culture Rubus glaucus Benth Glomus sp. (GEV02) in vitro mycorrhization. |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/3688/2752 |
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