TROUBLESHOOTING IN EXPRESSION AND PURIFICATION OF RECOMBINANT SEVERE ACUTE RESPIRATORY SYNDROME-ASSOCIATED CORONAVIRUS NUCLEOCAPSID PROTEIN IN Escherichia coli BL21

TROUBLESHOOTING IN EXPRESSION AND PURIFICATION OF RECOMBINANT SEVERE ACUTE RESPIRATORY SYNDROME-ASSOCIATED CORONAVIRUS NUCLEOCAPSID PROTEIN IN Escherichia coli BL21

Considering importance of N protein for study of viral pathogenesis or development of immunodiagnostic assay, wereported effects of several conditions on purity and homogeneity of recombinant SARS-CoV N protein expressed in E.coli BL21. The SARS-CoV N gene was reverse transcribed and amplified by th...

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Bibliographic Details
Main Authors: Budiman Bela, Fera Ibrahim, Andi Yasmon
Format: Article
Language:English
Published: Universitas Indonesia 2010-11-01
Series:Makara Seri Sains
Subjects:
imidazol
IPTG
N-lauroylsarcosine
Triton X
SARS-CoV N protein
Online Access:http://journal.ui.ac.id/science/article/viewFile/741/704

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