Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV)
Research has shown that long-term exposure to lead harms the hematological system. The homeostatic iron regulator <i>HFE</i> (hemochromatosis) mutation, which has been shown to affect iron absorption and iron overload, is hypothesized to be related to lead intoxication in vulnerable indi...
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doaj-967f6f0165ed4a35b6c6d8b20c65e93b2020-11-24T20:47:25ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012019-01-0116335410.3390/ijerph16030354ijerph16030354Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV)Chien-Juan Chen0Ting-Yi Lin1Chao-Ling Wang2Chi-Kung Ho3Hung-Yi Chuang4Hsin-Su Yu5Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanMaster Program of Public Health, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Environmental and Occupational Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, TaiwanDepartment of Environmental and Occupational Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanResearch has shown that long-term exposure to lead harms the hematological system. The homeostatic iron regulator <i>HFE</i> (hemochromatosis) mutation, which has been shown to affect iron absorption and iron overload, is hypothesized to be related to lead intoxication in vulnerable individuals. The aim of our study was to investigate whether the <i>HFE</i> genotype modifies the blood lead levels that affect the distributions of serum iron and other red blood cell indices. Overall, 121 lead workers and 117 unexposed age-matched subjects were recruited for the study. The collected data included the blood lead levels, complete blood count, serum iron, total iron binding capacity, transferrin, and ferritin, which were measured during regular physical examinations. All subjects filled out questionnaires that included demographic information, medical history, and alcohol and tobacco consumption. <i>HFE</i> genotyping for C282Y and H63D was determined using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). The mean blood lead level in lead workers was 19.75 µg/dL and was 2.86 µg/dL in unexposed subjects. Of 238 subjects, 221 (92.9%) subjects were wild-type (CCHH) for <i>HFE</i> C282Y and H63D, and 17 (7.1%) subjects were heterozygous for a H63D mutation (CCHD). Multiple linear regression analysis showed that blood lead was significantly negatively associated with hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular volume (MCV), whereas the <i>HFE</i> variant was associated negatively with MCV and positively with ferritin. An interactive influence on MCV was identified between blood lead and <i>HFE</i> variants. Our research found a significant modifying effect of the <i>HFE</i> variant, which possibly affected MCV. The <i>HFE</i> H63D heterozygous (CCHD) variant seemed to provide a protective factor against lead toxicity. Future studies should focus on competing binding proteins between iron and lead influenced by gene variation.https://www.mdpi.com/1660-4601/16/3/354leadhemochromatosis<i>HFE</i>ferritinmean corpuscular volume MCV |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-Juan Chen Ting-Yi Lin Chao-Ling Wang Chi-Kung Ho Hung-Yi Chuang Hsin-Su Yu |
spellingShingle |
Chien-Juan Chen Ting-Yi Lin Chao-Ling Wang Chi-Kung Ho Hung-Yi Chuang Hsin-Su Yu Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) International Journal of Environmental Research and Public Health lead hemochromatosis <i>HFE</i> ferritin mean corpuscular volume MCV |
author_facet |
Chien-Juan Chen Ting-Yi Lin Chao-Ling Wang Chi-Kung Ho Hung-Yi Chuang Hsin-Su Yu |
author_sort |
Chien-Juan Chen |
title |
Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) |
title_short |
Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) |
title_full |
Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) |
title_fullStr |
Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) |
title_full_unstemmed |
Interactive Effects between Chronic Lead Exposure and the Homeostatic Iron Regulator Transport <i>HFE</i> Polymorphism on the Human Red Blood Cell Mean Corpuscular Volume (MCV) |
title_sort |
interactive effects between chronic lead exposure and the homeostatic iron regulator transport <i>hfe</i> polymorphism on the human red blood cell mean corpuscular volume (mcv) |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2019-01-01 |
description |
Research has shown that long-term exposure to lead harms the hematological system. The homeostatic iron regulator <i>HFE</i> (hemochromatosis) mutation, which has been shown to affect iron absorption and iron overload, is hypothesized to be related to lead intoxication in vulnerable individuals. The aim of our study was to investigate whether the <i>HFE</i> genotype modifies the blood lead levels that affect the distributions of serum iron and other red blood cell indices. Overall, 121 lead workers and 117 unexposed age-matched subjects were recruited for the study. The collected data included the blood lead levels, complete blood count, serum iron, total iron binding capacity, transferrin, and ferritin, which were measured during regular physical examinations. All subjects filled out questionnaires that included demographic information, medical history, and alcohol and tobacco consumption. <i>HFE</i> genotyping for C282Y and H63D was determined using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). The mean blood lead level in lead workers was 19.75 µg/dL and was 2.86 µg/dL in unexposed subjects. Of 238 subjects, 221 (92.9%) subjects were wild-type (CCHH) for <i>HFE</i> C282Y and H63D, and 17 (7.1%) subjects were heterozygous for a H63D mutation (CCHD). Multiple linear regression analysis showed that blood lead was significantly negatively associated with hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular volume (MCV), whereas the <i>HFE</i> variant was associated negatively with MCV and positively with ferritin. An interactive influence on MCV was identified between blood lead and <i>HFE</i> variants. Our research found a significant modifying effect of the <i>HFE</i> variant, which possibly affected MCV. The <i>HFE</i> H63D heterozygous (CCHD) variant seemed to provide a protective factor against lead toxicity. Future studies should focus on competing binding proteins between iron and lead influenced by gene variation. |
topic |
lead hemochromatosis <i>HFE</i> ferritin mean corpuscular volume MCV |
url |
https://www.mdpi.com/1660-4601/16/3/354 |
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