Comparative Serological and Random Amplified Polymorphic DNA Typing for Bordetella avium Isolates in China

• Main Author: Ping-Ping Yang, Rong-De Ma1, Xue Zhao and Rui-Liang Zhu*
• Format: Article
• Language: English
• Published: University of Agriculture, Faisalabad 2012-10-01
• Series: Pakistan Veterinary Journal
• Subjects: Bordetella avium; Plate-agglutination test; RAPD typing; Serotyping
• Online Access: http://pvj.com.pk/pdf-files/32_4/552-556.pdf

To study the similarity among Bordetella avium isolates in China, antigens and diagnostic antiserum of 22 B. avium isolates were prepared for serotyping, and a set of 20 commercially available primers was screened out to identify suitable primers for random amplified polymorphic DNA fingerprinting (RAPD) analysis in this study. Twenty-two B. avium isolates were divided into two serovars (A and B) based on their reaction in the plate-agglutination test. Four primers R1, R2, R4 and R10 resulted in informative fingerprints and were used to evaluate the B. avium isolates. Based on their RAPD patterns, a dendrogram allowed the separation of the B. avium isolates into six genetic similarity clusters. However, no direct correlation was observed between serotypes and RAPD typing among the isolates.