Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration
Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO<sub>2</sub> supply can also be used to improve both lipids and carbohydrat...
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doaj-96a5e56266cd4757b24812d1d30bcb072020-11-25T01:36:22ZengMDPI AGBiology2079-77372019-12-0191510.3390/biology9010005biology9010005Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> ConcentrationErik L. Jensen0Karen Yangüez1Frédéric Carrière2Brigitte Gontero3CNRS, BIP, UMR 7281, IMM, FR3479, 31 Chemin J. Aiguier, Aix Marseille Univ., 13 402 Marseille CEDEX 20, FranceCNRS, BIP, UMR 7281, IMM, FR3479, 31 Chemin J. Aiguier, Aix Marseille Univ., 13 402 Marseille CEDEX 20, FranceCNRS, BIP, UMR 7281, IMM, FR3479, 31 Chemin J. Aiguier, Aix Marseille Univ., 13 402 Marseille CEDEX 20, FranceCNRS, BIP, UMR 7281, IMM, FR3479, 31 Chemin J. Aiguier, Aix Marseille Univ., 13 402 Marseille CEDEX 20, FranceAccumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO<sub>2</sub> supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the two marine model diatoms—<i>Thalassiosira pseudonana</i> and <i>Phaeodactylum tricornutum</i>, a freshwater diatom, <i>Asterionella formosa</i>, and <i>Navicula pelliculosa</i>—found in fresh- and sea-water environments. We measured the accumulation of reserve compounds and the activity of enzymes involved in carbon metabolism in these diatoms grown at high and atmospheric CO<sub>2</sub>. We observed that biomass and lipid accumulation in cells grown at high CO<sub>2</sub> differ among the diatoms. Lipid accumulation increased only in <i>P. tricornutum</i> and <i>N. pelliculosa</i> grown in seawater in response to elevated CO<sub>2</sub>. Moreover, accumulation of lipids was also accompanied by an increased activity of the enzymes tested. However, lipid accumulation and enzyme activity decreased in <i>N. pelliculosa</i> cultured in fresh water. Chrysolaminarin accumulation was also affected by CO<sub>2</sub> concentration; however, there was no clear relation with lipids accumulation. Our results are relevant to understand better the ecological role of the environment in the diatom adaptation to CO<sub>2</sub> and the mechanisms underpinning the production of storage compounds considering diatom diversity.https://www.mdpi.com/2079-7737/9/1/5co<sub>2</sub>chrysolaminarindiatomstriacylglycerol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erik L. Jensen Karen Yangüez Frédéric Carrière Brigitte Gontero |
spellingShingle |
Erik L. Jensen Karen Yangüez Frédéric Carrière Brigitte Gontero Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration Biology co<sub>2</sub> chrysolaminarin diatoms triacylglycerol |
author_facet |
Erik L. Jensen Karen Yangüez Frédéric Carrière Brigitte Gontero |
author_sort |
Erik L. Jensen |
title |
Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration |
title_short |
Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration |
title_full |
Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration |
title_fullStr |
Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration |
title_full_unstemmed |
Storage Compound Accumulation in Diatoms as Response to Elevated CO<sub>2</sub> Concentration |
title_sort |
storage compound accumulation in diatoms as response to elevated co<sub>2</sub> concentration |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2019-12-01 |
description |
Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO<sub>2</sub> supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the two marine model diatoms—<i>Thalassiosira pseudonana</i> and <i>Phaeodactylum tricornutum</i>, a freshwater diatom, <i>Asterionella formosa</i>, and <i>Navicula pelliculosa</i>—found in fresh- and sea-water environments. We measured the accumulation of reserve compounds and the activity of enzymes involved in carbon metabolism in these diatoms grown at high and atmospheric CO<sub>2</sub>. We observed that biomass and lipid accumulation in cells grown at high CO<sub>2</sub> differ among the diatoms. Lipid accumulation increased only in <i>P. tricornutum</i> and <i>N. pelliculosa</i> grown in seawater in response to elevated CO<sub>2</sub>. Moreover, accumulation of lipids was also accompanied by an increased activity of the enzymes tested. However, lipid accumulation and enzyme activity decreased in <i>N. pelliculosa</i> cultured in fresh water. Chrysolaminarin accumulation was also affected by CO<sub>2</sub> concentration; however, there was no clear relation with lipids accumulation. Our results are relevant to understand better the ecological role of the environment in the diatom adaptation to CO<sub>2</sub> and the mechanisms underpinning the production of storage compounds considering diatom diversity. |
topic |
co<sub>2</sub> chrysolaminarin diatoms triacylglycerol |
url |
https://www.mdpi.com/2079-7737/9/1/5 |
work_keys_str_mv |
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