In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications
Abstract Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO2) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applic...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Springer
2021-08-01
|
Series: | Journal of Materials Science: Materials in Medicine |
Online Access: | https://doi.org/10.1007/s10856-021-06596-6 |
id |
doaj-96a5ed0adc3448a38512b379d9ca0dbf |
---|---|
record_format |
Article |
spelling |
doaj-96a5ed0adc3448a38512b379d9ca0dbf2021-09-05T11:39:08ZengSpringerJournal of Materials Science: Materials in Medicine0957-45301573-48382021-08-0132911010.1007/s10856-021-06596-6In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applicationsC. C. Wachesk0S. H. Seabra1T. A. T. Dos Santos2V. J. Trava-Airoldi3A. O. Lobo4F. R. Marciano5Laboratory of Nanotechnology and Toxicology, Department of Science and Technology, UNIFESP—Federal University of São PauloTechnology Laboratory of Biochemistry and Microscopy, UEZO—Universidade Estadual da Zona OesteTechnology Laboratory of Biochemistry and Microscopy, UEZO—Universidade Estadual da Zona OesteAssociated Laboratory of Sensors and Materials, INPE—National Institute for Space ResearchLIMAV-Interdisciplinary Laboratory for Advanced Materials, Materials Science & Engineering Graduate Program, UFPI—Federal University of PiauiDepartment of Physics, UFPI—Federal University of PiauiAbstract Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO2) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applications in biomedical engineering. Here we investigate for the first time the in vivo inflammatory process of DLC coatings with incorporated TiO2 nanoparticles. TiO2-DLC films were grown on AISI 316 stainless-steel substrates using plasma-enhanced chemical vapor deposition. The coated substrates were implanted in CF1 mice peritoneum. The in vivo cytotoxicity and biocompatibility of the samples were analyzed from macrophage lavage. Analysis in the first weeks after implantation could be helpful to evaluate the acute cytotoxicity generated after a possible inflammatory process. The in vivo results showed no inflammatory process. A significant increase in nitric oxide production on the uncoated substrates was confirmed through cytometry, and the coated substrates demonstrated biocompatibility. The presence of TiO2 nanoparticles enhanced the wound healing activity, due to their astringent and antimicrobial properties. DLC and TiO2-DLC coatings were considered biocompatible, and the presence of TiO2 nanoparticles reduced the inflammatory reactions, increasing DLC biocompatibility.https://doi.org/10.1007/s10856-021-06596-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C. C. Wachesk S. H. Seabra T. A. T. Dos Santos V. J. Trava-Airoldi A. O. Lobo F. R. Marciano |
spellingShingle |
C. C. Wachesk S. H. Seabra T. A. T. Dos Santos V. J. Trava-Airoldi A. O. Lobo F. R. Marciano In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications Journal of Materials Science: Materials in Medicine |
author_facet |
C. C. Wachesk S. H. Seabra T. A. T. Dos Santos V. J. Trava-Airoldi A. O. Lobo F. R. Marciano |
author_sort |
C. C. Wachesk |
title |
In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications |
title_short |
In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications |
title_full |
In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications |
title_fullStr |
In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications |
title_full_unstemmed |
In vivo biocompatibility of diamond-like carbon films containing TiO2 nanoparticles for biomedical applications |
title_sort |
in vivo biocompatibility of diamond-like carbon films containing tio2 nanoparticles for biomedical applications |
publisher |
Springer |
series |
Journal of Materials Science: Materials in Medicine |
issn |
0957-4530 1573-4838 |
publishDate |
2021-08-01 |
description |
Abstract Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO2) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applications in biomedical engineering. Here we investigate for the first time the in vivo inflammatory process of DLC coatings with incorporated TiO2 nanoparticles. TiO2-DLC films were grown on AISI 316 stainless-steel substrates using plasma-enhanced chemical vapor deposition. The coated substrates were implanted in CF1 mice peritoneum. The in vivo cytotoxicity and biocompatibility of the samples were analyzed from macrophage lavage. Analysis in the first weeks after implantation could be helpful to evaluate the acute cytotoxicity generated after a possible inflammatory process. The in vivo results showed no inflammatory process. A significant increase in nitric oxide production on the uncoated substrates was confirmed through cytometry, and the coated substrates demonstrated biocompatibility. The presence of TiO2 nanoparticles enhanced the wound healing activity, due to their astringent and antimicrobial properties. DLC and TiO2-DLC coatings were considered biocompatible, and the presence of TiO2 nanoparticles reduced the inflammatory reactions, increasing DLC biocompatibility. |
url |
https://doi.org/10.1007/s10856-021-06596-6 |
work_keys_str_mv |
AT ccwachesk invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications AT shseabra invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications AT tatdossantos invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications AT vjtravaairoldi invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications AT aolobo invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications AT frmarciano invivobiocompatibilityofdiamondlikecarbonfilmscontainingtio2nanoparticlesforbiomedicalapplications |
_version_ |
1717814081957658624 |