MiR-217 is involved in the carcinogenesis of gastric cancer by down-regulating CDH1 expression

• Main Authors: Wei Li, Yu-Qiang Gao
• Format: Article
• Language: English
• Published: Wiley 2018-07-01
• Series: Kaohsiung Journal of Medical Sciences
• Online Access: http://www.sciencedirect.com/science/article/pii/S1607551X17307398

GC is one of the most leading malignancies all over the world, and is also the leading cause of cancer-related mortalities. At present, GC remains difficult to diagnose at an early stage. In this study, we first detected the expression of 9 selected miRNAs in the exosomes from 67 GC patients’ circular exosomes and found 4 miRNAs level was significantly altered. Meanwhile, one out of 4 candidate miRNAs also had a higher expression in the GC tissue samples, and negative correlated with CDH1 expression. Predicted by bioinformatics tools, confirmed by dual luciferase assay and immunoblotting, we identified that CDH1 is a direct target of miR-217. MiR-217 overexpression enhanced gastric cancer cells proliferation, and reduced exosomal CDH1 level which can be delivered into microenvironment. In conclusion, we constructed the negative correlation between miR-217 and CDH1 level in GC patients and cells; unveiled part of the miR-217 function during the pathogenesis of GC. These findings may give insight into understanding the mechanism of GC pathogenesis and provide new biomarkers for clinical diagnosis. Keywords: Gastric cancer, miRNA, Exosome, CDH1