Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches
The potential probiotic benefits of <i>Lactobacillus mucosae</i> have received increasing attention. To investigate the genetic diversity of <i>L. mucosae</i>, comparative genomic analyses of 93 strains isolated from different niches (human and animal gut, human vagina, etc.)...
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doaj-96c1952a0e1a4e67b3782747e49423ba2020-11-25T00:30:35ZengMDPI AGGenes2073-44252020-01-011119510.3390/genes11010095genes11010095Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different NichesYan Jia0Bo Yang1Paul Ross2Catherine Stanton3Hao Zhang4Jianxin Zhao5Wei Chen6State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaThe potential probiotic benefits of <i>Lactobacillus mucosae</i> have received increasing attention. To investigate the genetic diversity of <i>L. mucosae</i>, comparative genomic analyses of 93 strains isolated from different niches (human and animal gut, human vagina, etc.) and eight strains of published genomes were conducted. The results showed that the core genome of <i>L. mucosae</i> mainly encoded translation and transcription, amino acid biosynthesis, sugar metabolism, and defense function while the pan-genomic curve tended to be close. The genetic diversity of <i>L. mucosae</i> mainly reflected in carbohydrate metabolism and immune/competitive-related factors, such as exopolysaccharide (EPS), enterolysin A, and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas. It was worth noting that this research firstly predicted the complete EPS operon shared among <i>L. mucosae</i>. Additionally, the type IIIA CRISPR-Cas system was discovered in <i>L. mucosae</i> for the first time. This work provided new ideas for the study of this species.https://www.mdpi.com/2073-4425/11/1/95<i>lactobacillus mucosae</i>comparative genomicsenterolysin acarbohydratecrispr-caseps |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Jia Bo Yang Paul Ross Catherine Stanton Hao Zhang Jianxin Zhao Wei Chen |
spellingShingle |
Yan Jia Bo Yang Paul Ross Catherine Stanton Hao Zhang Jianxin Zhao Wei Chen Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches Genes <i>lactobacillus mucosae</i> comparative genomics enterolysin a carbohydrate crispr-cas eps |
author_facet |
Yan Jia Bo Yang Paul Ross Catherine Stanton Hao Zhang Jianxin Zhao Wei Chen |
author_sort |
Yan Jia |
title |
Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches |
title_short |
Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches |
title_full |
Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches |
title_fullStr |
Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches |
title_full_unstemmed |
Comparative Genomics Analysis of <i>Lactobacillus mucosae</i> from Different Niches |
title_sort |
comparative genomics analysis of <i>lactobacillus mucosae</i> from different niches |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-01-01 |
description |
The potential probiotic benefits of <i>Lactobacillus mucosae</i> have received increasing attention. To investigate the genetic diversity of <i>L. mucosae</i>, comparative genomic analyses of 93 strains isolated from different niches (human and animal gut, human vagina, etc.) and eight strains of published genomes were conducted. The results showed that the core genome of <i>L. mucosae</i> mainly encoded translation and transcription, amino acid biosynthesis, sugar metabolism, and defense function while the pan-genomic curve tended to be close. The genetic diversity of <i>L. mucosae</i> mainly reflected in carbohydrate metabolism and immune/competitive-related factors, such as exopolysaccharide (EPS), enterolysin A, and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas. It was worth noting that this research firstly predicted the complete EPS operon shared among <i>L. mucosae</i>. Additionally, the type IIIA CRISPR-Cas system was discovered in <i>L. mucosae</i> for the first time. This work provided new ideas for the study of this species. |
topic |
<i>lactobacillus mucosae</i> comparative genomics enterolysin a carbohydrate crispr-cas eps |
url |
https://www.mdpi.com/2073-4425/11/1/95 |
work_keys_str_mv |
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1725325950526160896 |