Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles
Abstract Background Humans are continuously exposed to particles in the gastrointestinal tract. Exposure may occur directly through ingestion of particles via food or indirectly by removal of inhaled material from the airways by the mucociliary clearance system. We examined the effects of food-grade...
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doaj-96e505bacf1c4836adf1d64acdb952dc2020-11-25T01:09:46ZengBMCParticle and Fibre Toxicology1743-89772018-02-0115111810.1186/s12989-018-0248-2Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particlesDitte Marie Jensen0Daniel Vest Christophersen1Majid Sheykhzade2Gry Freja Skovsted3Jens Lykkesfeldt4Rasmus Münter5Martin Roursgaard6Steffen Loft7Peter Møller8Department of Public Health, Section of Environmental Health, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Public Health, Section of Environmental Health, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Drug Design and Pharmacology, Section of Molecular and Cellular Pharmacology, Faculty of Health and Medical Sciences, University of CopenhagenExperimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenExperimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenColloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of DenmarkDepartment of Public Health, Section of Environmental Health, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Public Health, Section of Environmental Health, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Public Health, Section of Environmental Health, Faculty of Health and Medical Sciences, University of CopenhagenAbstract Background Humans are continuously exposed to particles in the gastrointestinal tract. Exposure may occur directly through ingestion of particles via food or indirectly by removal of inhaled material from the airways by the mucociliary clearance system. We examined the effects of food-grade particle exposure on vasomotor function and systemic oxidative stress in an ex vivo study and intragastrically exposed rats. Methods In an ex vivo study, aorta rings from naïve Sprague-Dawley rats were exposed for 30 min to food-grade TiO2 (E171), benchmark TiO2 (Aeroxide P25), food-grade vegetable carbon (E153) or benchmark carbon black (Printex 90). Subsequently, the vasomotor function was assessed in wire myographs. In an in vivo study, lean Zucker rats were exposed intragastrically once a week for 10 weeks to vehicle, E171 or E153. Doses were comparable to human daily intake. Vasomotor function in the coronary arteries and aorta was assessed using wire myographs. Tetrahydrobiopterin, ascorbate, malondialdehyde and asymmetric dimethylarginine were measured in blood as markers of oxidative stress and vascular function. Results Direct exposure of E171 to aorta rings ex vivo increased the acetylcholine-induced vasorelaxation and 5-hydroxytryptamine-induced vasocontraction. E153 only increased acetylcholine-induced vasorelaxation, and Printex 90 increased the 5-hydroxytryptamine-induced vasocontraction, whereas Aeroxide P25 did not affect the vasomotor function. In vivo exposure showed similar results as ex vivo exposure; increased acetylcholine-induced vasorelaxation in coronary artery segments of E153 and E171 exposed rats, whereas E171 exposure altered 5-hydroxytryptamine-induced vasocontraction in distal coronary artery segments. Plasma levels of markers of oxidative stress and vascular function showed no differences between groups. Conclusion Gastrointestinal tract exposure to E171 and E153 was associated with modest albeit statistically significant alterations in the vasocontraction and vasorelaxation responses. Direct particle exposure to aorta rings elicited a similar type of response. The vasomotor responses were not related to biomarkers of systemic oxidative stress.http://link.springer.com/article/10.1186/s12989-018-0248-2Vasomotor functionE153E171Vegetable carbonTitanium dioxideNanoparticles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ditte Marie Jensen Daniel Vest Christophersen Majid Sheykhzade Gry Freja Skovsted Jens Lykkesfeldt Rasmus Münter Martin Roursgaard Steffen Loft Peter Møller |
spellingShingle |
Ditte Marie Jensen Daniel Vest Christophersen Majid Sheykhzade Gry Freja Skovsted Jens Lykkesfeldt Rasmus Münter Martin Roursgaard Steffen Loft Peter Møller Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles Particle and Fibre Toxicology Vasomotor function E153 E171 Vegetable carbon Titanium dioxide Nanoparticles |
author_facet |
Ditte Marie Jensen Daniel Vest Christophersen Majid Sheykhzade Gry Freja Skovsted Jens Lykkesfeldt Rasmus Münter Martin Roursgaard Steffen Loft Peter Møller |
author_sort |
Ditte Marie Jensen |
title |
Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
title_short |
Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
title_full |
Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
title_fullStr |
Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
title_full_unstemmed |
Vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
title_sort |
vasomotor function in rat arteries after ex vivo and intragastric exposure to food-grade titanium dioxide and vegetable carbon particles |
publisher |
BMC |
series |
Particle and Fibre Toxicology |
issn |
1743-8977 |
publishDate |
2018-02-01 |
description |
Abstract Background Humans are continuously exposed to particles in the gastrointestinal tract. Exposure may occur directly through ingestion of particles via food or indirectly by removal of inhaled material from the airways by the mucociliary clearance system. We examined the effects of food-grade particle exposure on vasomotor function and systemic oxidative stress in an ex vivo study and intragastrically exposed rats. Methods In an ex vivo study, aorta rings from naïve Sprague-Dawley rats were exposed for 30 min to food-grade TiO2 (E171), benchmark TiO2 (Aeroxide P25), food-grade vegetable carbon (E153) or benchmark carbon black (Printex 90). Subsequently, the vasomotor function was assessed in wire myographs. In an in vivo study, lean Zucker rats were exposed intragastrically once a week for 10 weeks to vehicle, E171 or E153. Doses were comparable to human daily intake. Vasomotor function in the coronary arteries and aorta was assessed using wire myographs. Tetrahydrobiopterin, ascorbate, malondialdehyde and asymmetric dimethylarginine were measured in blood as markers of oxidative stress and vascular function. Results Direct exposure of E171 to aorta rings ex vivo increased the acetylcholine-induced vasorelaxation and 5-hydroxytryptamine-induced vasocontraction. E153 only increased acetylcholine-induced vasorelaxation, and Printex 90 increased the 5-hydroxytryptamine-induced vasocontraction, whereas Aeroxide P25 did not affect the vasomotor function. In vivo exposure showed similar results as ex vivo exposure; increased acetylcholine-induced vasorelaxation in coronary artery segments of E153 and E171 exposed rats, whereas E171 exposure altered 5-hydroxytryptamine-induced vasocontraction in distal coronary artery segments. Plasma levels of markers of oxidative stress and vascular function showed no differences between groups. Conclusion Gastrointestinal tract exposure to E171 and E153 was associated with modest albeit statistically significant alterations in the vasocontraction and vasorelaxation responses. Direct particle exposure to aorta rings elicited a similar type of response. The vasomotor responses were not related to biomarkers of systemic oxidative stress. |
topic |
Vasomotor function E153 E171 Vegetable carbon Titanium dioxide Nanoparticles |
url |
http://link.springer.com/article/10.1186/s12989-018-0248-2 |
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