Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines
Abstract Background An extract of Ficus religiosa latex has been previously found to possess potent pharmacological activity with high antioxidant content phytochemical. The present research was conducted to investigate the chemo-preventive efficacy of latex extract on human breast adenocarcinoma MD...
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doaj-96fe4104c8974532ace7dee8f5b80dec2021-02-07T12:46:36ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532021-02-017112110.1186/s43094-021-00182-xEvaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell linesL. Saida0C. D. S. L. N. Tulasi1M. Lakshmi Narasu2Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological InstituteCentre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological InstituteCentre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological InstituteAbstract Background An extract of Ficus religiosa latex has been previously found to possess potent pharmacological activity with high antioxidant content phytochemical. The present research was conducted to investigate the chemo-preventive efficacy of latex extract on human breast adenocarcinoma MDA MB 231, human neroblastoma IMR 32, and human colorectal HCT 116 cell lines. Results The results showed that the latex crude extract induced cytotoxicity in all the selected cell lines with IC50 value 4.8 ± 1.13 μg/ml against the IMR 32 cell line. The cell cycle analysis results indicated the arrest and accumulation of cells at G1 phase in case of MDA MB 231 cells and HCT 116 cells whereas in the case of IMR 32 cells the arrest was in G2/M phase. The clear bands of fragments observed in DNA ladder experiments showed that apoptosis is induced by extracts in the cell lines. This could be correlated with the gene level expression studies on selected pro-apoptotic (p53 and caspase-3) and anti-apoptotic (Bcl-2, AKT) genes, which got upregulated and downregulated, respectively. Conclusion Based on the experimental evidence, Ficus religiosa contains phytochemicals with potent antitumor activities.https://doi.org/10.1186/s43094-021-00182-xFicus religiosaCytotoxicityApoptosisCell cycle arrest |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
L. Saida C. D. S. L. N. Tulasi M. Lakshmi Narasu |
spellingShingle |
L. Saida C. D. S. L. N. Tulasi M. Lakshmi Narasu Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines Future Journal of Pharmaceutical Sciences Ficus religiosa Cytotoxicity Apoptosis Cell cycle arrest |
author_facet |
L. Saida C. D. S. L. N. Tulasi M. Lakshmi Narasu |
author_sort |
L. Saida |
title |
Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines |
title_short |
Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines |
title_full |
Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines |
title_fullStr |
Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines |
title_full_unstemmed |
Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines |
title_sort |
evaluation of chemo-preventive efficacy of ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by rt-pcr in various cell lines |
publisher |
SpringerOpen |
series |
Future Journal of Pharmaceutical Sciences |
issn |
2314-7253 |
publishDate |
2021-02-01 |
description |
Abstract Background An extract of Ficus religiosa latex has been previously found to possess potent pharmacological activity with high antioxidant content phytochemical. The present research was conducted to investigate the chemo-preventive efficacy of latex extract on human breast adenocarcinoma MDA MB 231, human neroblastoma IMR 32, and human colorectal HCT 116 cell lines. Results The results showed that the latex crude extract induced cytotoxicity in all the selected cell lines with IC50 value 4.8 ± 1.13 μg/ml against the IMR 32 cell line. The cell cycle analysis results indicated the arrest and accumulation of cells at G1 phase in case of MDA MB 231 cells and HCT 116 cells whereas in the case of IMR 32 cells the arrest was in G2/M phase. The clear bands of fragments observed in DNA ladder experiments showed that apoptosis is induced by extracts in the cell lines. This could be correlated with the gene level expression studies on selected pro-apoptotic (p53 and caspase-3) and anti-apoptotic (Bcl-2, AKT) genes, which got upregulated and downregulated, respectively. Conclusion Based on the experimental evidence, Ficus religiosa contains phytochemicals with potent antitumor activities. |
topic |
Ficus religiosa Cytotoxicity Apoptosis Cell cycle arrest |
url |
https://doi.org/10.1186/s43094-021-00182-x |
work_keys_str_mv |
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