Immune co-culture cell microarray – a feasible tool for high-throughput functional investigation of lymphocyte–cancer interactions

• Main Authors: Erez Nissim Baruch, Rona Ortenberg, Camila Avivi, Liat Anafi, Daniela Dick-Necula, Chani Stossel, Yonatan Moshkovits, Orit Itzhaki, Michal Judith Besser, Jacob Schachter, Iris Barshack, Gal Markel
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2020-01-01
• Series: OncoImmunology
• Subjects: immunotherapy; melanoma; immunological cytotoxicity test; functional protein expression; omics validation
• Online Access: http://dx.doi.org/10.1080/2162402X.2020.1741267

Omics analyses often result in dozens to hundreds of potential targets, requiring validation for their biological relevance. Current high-throughput functional investigation methods are frequently labor-intensive, expensive, and display low reproducibility. The Immune Co-Culture Cell Microarray (ICCM) is a formalin-fixed paraffin-embedded cell block microarray based on co-cultures of patient-derived tumor-infiltrating lymphocytes and their autologous melanoma cells. Each ICCM slide represents the same experiment and can be stained using standard immunohistochemistry and immunofluorescence techniques. Functional dynamics assessment of both proteins and microRNAs using ICCM stained slides demonstrated similar findings to flow cytometry assays and to previously published patient-derived biopsy reports.