Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model
Background: The methanolic extract of Convolvulus pluricaulis had earlier shown lipid lowering activity in Triton induced reversible hyperlipidemia model, but, the hypolipidemic activity in irreversible models and hypoglycaemic activity are not investigated so far. Objective: This study was designed...
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doaj-970b6f4e0f2146979cc65420e4021c042020-12-25T05:06:11ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762020-10-01114426431Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat modelGaurav Garg0Amol N. Patil1Rohit Kumar2Alka Bhatia3Kripa Shanker Kasudhan4Smita Pattanaik5Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India; Corresponding author.Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Experimental Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, IndiaDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaDepartment of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, IndiaBackground: The methanolic extract of Convolvulus pluricaulis had earlier shown lipid lowering activity in Triton induced reversible hyperlipidemia model, but, the hypolipidemic activity in irreversible models and hypoglycaemic activity are not investigated so far. Objective: This study was designed to validate the lipid and glucose-lowering actions of C. pluricaulis methanolic extract (CPME) by using ingredients from the Indian diet for induction of hyperlipidemia and diabetes on experimental rats. Materials and methods: Experimental animals were divided into four groups having six animals in each group (n = 6). Animals of Group I II, III and IV received - no treatment, 0.9% NaCl, Glipizide (GPZ) 5 mg/kg and CPME 400 mg/kg once daily for two weeks respectively. Animals of all groups except group I were fed a high fat-based Indian diet for 21 days followed by a single STZ (35 mg/kg) i.p. administration in model induction phase. Afterwards, animals were sacrificed, and the pancreas was dissected for histological changes, and blood was collected for measuring lipid parameters, FBS, insulin levels, and HOMA scores. Results: CPME significantly ameliorate the lipid abnormalities in HFD-STZ-treated experimental model (p < 0.001) but fails to reverse the hyperglycaemia developed in diabetic rats with no protective effect on islet architecture (p > 0.05) as compared to experimental group while, GPZ showed protective effect on both lipid abnormalities and hyperglycemia by modulating the levels of lipid parameters and insulin respectively. Conclusion: In conclusion, the study confirm that CPME possesses significant hypolipidemic activity but fails to reverse the hyperglycaemia developed in diabetic rats.http://www.sciencedirect.com/science/article/pii/S097594761930052XConvolvulus pluricaulisStreptozotocinHypolipidemiaHigh fat diet |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaurav Garg Amol N. Patil Rohit Kumar Alka Bhatia Kripa Shanker Kasudhan Smita Pattanaik |
spellingShingle |
Gaurav Garg Amol N. Patil Rohit Kumar Alka Bhatia Kripa Shanker Kasudhan Smita Pattanaik Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model Journal of Ayurveda and Integrative Medicine Convolvulus pluricaulis Streptozotocin Hypolipidemia High fat diet |
author_facet |
Gaurav Garg Amol N. Patil Rohit Kumar Alka Bhatia Kripa Shanker Kasudhan Smita Pattanaik |
author_sort |
Gaurav Garg |
title |
Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
title_short |
Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
title_full |
Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
title_fullStr |
Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
title_full_unstemmed |
Protective role of Convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
title_sort |
protective role of convolvulus pluricaulis on lipid abnormalities in high-fat diet with low dose streptozotocin-induced experimental rat model |
publisher |
Elsevier |
series |
Journal of Ayurveda and Integrative Medicine |
issn |
0975-9476 |
publishDate |
2020-10-01 |
description |
Background: The methanolic extract of Convolvulus pluricaulis had earlier shown lipid lowering activity in Triton induced reversible hyperlipidemia model, but, the hypolipidemic activity in irreversible models and hypoglycaemic activity are not investigated so far. Objective: This study was designed to validate the lipid and glucose-lowering actions of C. pluricaulis methanolic extract (CPME) by using ingredients from the Indian diet for induction of hyperlipidemia and diabetes on experimental rats. Materials and methods: Experimental animals were divided into four groups having six animals in each group (n = 6). Animals of Group I II, III and IV received - no treatment, 0.9% NaCl, Glipizide (GPZ) 5 mg/kg and CPME 400 mg/kg once daily for two weeks respectively. Animals of all groups except group I were fed a high fat-based Indian diet for 21 days followed by a single STZ (35 mg/kg) i.p. administration in model induction phase. Afterwards, animals were sacrificed, and the pancreas was dissected for histological changes, and blood was collected for measuring lipid parameters, FBS, insulin levels, and HOMA scores. Results: CPME significantly ameliorate the lipid abnormalities in HFD-STZ-treated experimental model (p < 0.001) but fails to reverse the hyperglycaemia developed in diabetic rats with no protective effect on islet architecture (p > 0.05) as compared to experimental group while, GPZ showed protective effect on both lipid abnormalities and hyperglycemia by modulating the levels of lipid parameters and insulin respectively. Conclusion: In conclusion, the study confirm that CPME possesses significant hypolipidemic activity but fails to reverse the hyperglycaemia developed in diabetic rats. |
topic |
Convolvulus pluricaulis Streptozotocin Hypolipidemia High fat diet |
url |
http://www.sciencedirect.com/science/article/pii/S097594761930052X |
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