An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-κB as an example.
SELEX-Seq is now the optimal high-throughput technique for characterizing DNA-binding specificities of transcription factors. In this study, we introduced an improved EMSA-based SELEX-Seq strategy with several advantages. The improvements of this strategy included: (1) using a FAM-labeled probe to t...
Main Authors: | Guangming Gu, Tingting Wang, Yang Yang, Xinhui Xu, Jinke Wang |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3794954?pdf=render |
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